摘要
目的通过跟踪复查,摸索抗-TP单试剂反应性献血者的归队策略。方法对抗-TP单试剂反应性献血者进行多次跟踪复查,包括酶联免疫法(ELISA)和梅毒螺旋体抗体明胶颗粒凝集试验(TPPA),每次时间间隔≥3个月。献血者的复查判定:3种抗-TP检测试剂中≥2种呈反应性,献血者继续屏蔽,不再参与跟踪;其余的情况,献血者继续跟踪观察。结果2013年1月1日-2015年9月30日间,在415名抗-TP单试剂反应性献血者中有65人(15.66%)参与了研究,跟踪时间跨度95-892d(中位数371d)。全部跟踪检测的TPPA结果为无反应性,ELISA再次反应性的献血者有20人(30.77%):参与1次复查41人,再次反应性14人(34.15%);参与2次14人,再次反应性5人(35.71%);参与3次10人,再次反应性1人(10.00%);共有4人发生了跟踪结果反应性与无反应性交替出现的情况。结论抗-TP单试剂反应性献血者归队策略为:跟踪复查≥2次,每次时间间隔≥3个月,若末次结果中任意试剂出现反应性则继续屏蔽;若末次结果无反应性即可恢复献血资格。
Objective To propose an viable reentry strategy for single-reactive blood donors in Anti-TP ELISA tests.Methods Follow-up samples from research donors were collected according to a schedule with a 3-month interval. Reconfirmation tests were then performed on the collected samples using routine and supplementary tests( Enzyme-linked immunosorbent assay,ELISA; Treponema pallidum particle agglutination assay,TPPA). Should more than 1 reactive result appeared in the 3 tests( 2 ELISA kits and 1 TPPA kit),the donor is determined unqualified while other result cases require further follow-up. Results During January 1 st 2013 to September 30 th 2015,sixty-five of 415 blood donors were single-reactive in the Anti-TP ELISA tests and were therefore enrolled in this study. The follow-up periods ranged from 95 d-892 d( median 371 d). All follow-up samples’ TPPA results were non-reactive while reactive samples were detected in 20 of the 65( 30. 77%)donors. 41 of the qualified donors went through the follow-up retesting once,14 twice and 10 three-time. 14,5 and 1 samples were detected as reactive respectively. Conclusion The reentry of these single-reactive patients requires at least twice or more follow-up tests with an interval of more than 3 months. Any reactive results leads to further follow-ups. Only the all-clear donors could be assigned to reenter blood donation.
作者
王东
臧亮
陈辉
邓雪莲
梁晓华
WANG Dong;ZANG Liang;CHEN Hui;DENG Xuelian;LIANG Xiaohua(Liaoning Dalian Blood Center,Dalian 116001,China.)
出处
《中国输血杂志》
CAS
2018年第10期1177-1180,共4页
Chinese Journal of Blood Transfusion
基金
卫生部医药卫生科技发展研究中心专项课题(卫计中[2012]12号28-8-3)
关键词
梅毒螺旋体抗体
献血者归队
血清学检测
Treponema pallidum antibody
reentry of blood donors
Serological test.
