摘要
目的分离鉴定柘木中花旗松素、奥洛波尔、槲皮素3个黄酮类成分,并采用HPLC法测定其含量。方法采用柱层析法从柘木醇提物分离得到3种黄酮类成分,并通过核磁共振技术确定其结构。采用HPLC法检测其含量,色谱柱为Aglient ZORBAX SB-C18(4.6 mm×250 mm,5 μm),以1%醋酸-甲醇为流动相,梯度洗脱,流速1 ml/min,柱温25 ℃,检测波长310 nm。结果从柘木中分离鉴定出花旗松素、奥洛波尔、槲皮素3个黄酮类成分,其含量分别为0.850、0.518、0.103 mg/g。 结论本试验方法可为控制柘木药材的质量提供参考。
Objective To isolate and identify 3 flavonoids (taxifolin, orobol and quercetin) from Cudrania tricuspidata, and develop a method for determining 3 flavonoid constituents in Cudrania tricuspidata. Methods Three flavonoids was isolated from ethanol extract of Cudrania tricuspidata by chromatography, and its structure was identified by nuclear magnetic resonance. The analysis was conducted on an Aglient C18 column (4.6 mm ×250 mm, 5 μm) eluted with 1% acetic acid and methanol as mobile phases in gradient mode. The flow rate was 1 ml/min and the detection wavelength was set at 310 nm. The column temperature was 25 ℃. Results Taxifolin, orobol and quercetin were isolated from ethanol extract of Cudrania tricuspidata by chromatography. The content of taxifolin, orobol and quercetin were 0.850 mg/g, 0.518 mg/g, 0.103 mg/g. Conclusion The method can be used for the quality control of Cudrania tricuspidata as a reference.
作者
张伽妹
郭晓宇
全庆华
姬瑞芳
孙倩倩
田婧鋆
谭鹏
刘永刚
Zhang Jiamei;Guo Xiaoyu;Quan Qinghua;Ji Ruifang;Sun Qianqian;Tian Jingyun;Tan Peng;Liu Yonggang(Master of Grade 2017, Beijing University of Chinese Medicine, Beijng 100102, China)
出处
《国际中医中药杂志》
2018年第12期1187-1190,共4页
International Journal of Traditional Chinese Medicine
关键词
色谱法
高压液相
槲皮素
花旗松素
奥洛波尔
柘木
Chromatography,high pressure liquid
Quercetin
Taxifolin
Orobol
Cudrania tricuspidata
