摘要
目的探讨16SrDNA测序法用于胆道感染细菌病原体检测的可能性。方法采集潜在胆道感染患者胆汁标本,同时进行16SrDNA测序和常规培养,通过对比检测结果,评估16SrDNA测序用于临床实践的可行性。结果在19份标本中,2种方法均检出细菌的7份(36.84%),均未检测到细菌的7份(36.84%),16SrDNA测序阳性、培养阴性的5份(26.32%)。在2种方法同时检出细菌的标本中,结果高度相关的2份;与培养法相比,16SrDNA测序能够检出多种细菌,结果可被宏基因组测序部分证实。尽管2种方法检测结果在属水平具有一定相关性,但判断16S测序法检出的细菌与感染的关系仍存在困难。结论16SrDNA测序技术理论上具有高灵敏度、广覆盖等优点,随着测序和生物信息技术的发展,有望成为病原学诊断的辅助工具。
Objective To investigate the possibility of 16S rDNA sequencing in detecting bacterial pathogen of biliary tract infections. Methods Bile specimens from patients with potential biliary tract infections were collected for 16S rDNA sequencing and routine culture. The feasibility of 16S rDNA sequencing for clinical practice was evaluated by comparing the test results. Results In 19 samples,bacteria were detected from 7 samples( 36. 84%) by both methods and were not detected from 7 samples( 36. 84%) by both methods; 5 samples( 26. 32%) were 16S sequencing positive but culture negative. In the positive samples by both methods,only two cases showed a high level of result agreement; partial agreement and inconsistency between the two methods were seen in most of the samples. Furthermore,16S rDNA sequencing might detect more bacteria than culture, and the results were confirmed by the metagenomic sequencing. Although the detection results of the two methods had a certain correlation at the genus level,it was still difficult to judge the relationship between the bacteria detected by 16S sequencing method and infection. Conclusion Although the 16S rDNA sequencing is characterized by high sensitivity and wide coverage in theory,with the development of sequencing and bioinformatics technologies,16S rDNA sequencing shall be an aid to the diagnosis of pathogens.
作者
曹会玲
樊宁
宗春辉
崔云峰
刘运德
吴尚为
CAO Hui -ling;FAN Ning;ZONG Chun -hui;CUI Yun -feng;LIU Yun -de;WU Shang -wei(School of Laboratory Medicine,Tianjin Medical University,Tianjin 300203,China)
出处
《中国卫生检验杂志》
CAS
2018年第24期2987-2990,共4页
Chinese Journal of Health Laboratory Technology
