摘要
目的:比较4种煎药器皿对白芥子煎液中芥子碱含量及煎液特征图谱的影响,为经典名方的开发优选最佳的煎煮容器。方法:选择传统砂锅、陶瓷锅、圆底烧瓶、不锈钢锅4种煎煮器皿,以白芥子煎液中芥子碱含量及煎液的特征图谱为指标,探究不同煎煮器皿对白芥子指标性成分的含量和煎液特征图谱的影响。采用"中药色谱指纹图谱相似度评价系统"(2004A版)对特征图谱进行相似度评价。结果:传统砂锅、陶瓷锅、圆底烧瓶、不锈钢锅提取的芥子碱质量分数分别为0.04%,0.07%,0.84%,0.97%。传统砂锅与陶瓷锅、圆底烧瓶、不锈钢锅所制煎液的特征图谱相似度分别为0.98,0.82,0.68;陶瓷锅与圆底烧瓶、不锈钢锅所制煎液的特征图谱的相似度分别为0.79,0.62;圆底烧瓶与不锈钢锅所制煎液的特征图谱的相似度0.97。结论:不同煎煮容器所得白芥子煎液中芥子碱的含量有较大差异,传统砂锅与圆底烧瓶、不锈钢锅所得煎液的特征图谱的相似度较低。
Objective: To compare the effect of four kinds of decocting containers on the content of sinapine and the HPLC specific chromatograms of Sinapis Semen decoction,so as to optimize decocting container for the development of classical formulas. Method: Selecting four kinds of decoction vessels,named traditional casserole,ceramic pot,round-bottom flask and stainless-steel pot as the research object,the content of sinapine in Sinapis Semen decoction and its HPLC specific chromatograms were used as indexes to investigate the influence of different decoction vessels on the decoction. Similarity evaluation of specific chromatograms was performed by the " Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine"(edition of2004 A). Result: The contents of sinapine in the decoction prepared by traditional casserole,ceramic pot,roundbottom flask and stainless-steel pot were 0. 04%,0. 07%,0. 84% and 0. 97%,respectively. Compared with specific chromatograms of the decoction prepared by traditional casserole,the similarities of specific chromatograms of the decoction prepared by ceramic pot,round-bottom flask and stainless-steel pot were 0. 98,0. 82 and 0. 68,respectively. Compared with specific chromatograms of the decoction prepared by ceramic pot,the similarities of specific chromatograms of the decoction prepared by round-bottom flask and stainless-steel pot were 0. 79 and 0. 62,respectively. Compared with specific chromatograms of the decoction prepared by round-bottom flask, the similarity of specific chromatograms of the decoction prepared by stainless-steel pot was 0. 97. Conclusion: The content of sinapine and HPLC specific chromatograms of Sinapis Semen decoction obtained from different decocting containers are quite different.
作者
喻刚艳
薛宇涛
谭丽
喻祥龙
莫雨佳
罗菊元
陆洋
杜守颖
白洁
李鹏跃
YU Gang-yan;XUE Yu-tao;TAN Li;YU Xiang-long;MO Yu-jia;LUO Ju-yuan;LU Yang;DU Shou-ying;BAI Jie;LI Peng-yue(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100029,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2019年第1期183-188,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
北京中医药大学中央高校基本科研业务费专项(2018-JYB-XJQ004)
关键词
白芥子
煎煮容器
特征图谱
芥子碱硫氰酸盐
相似度
水煎液
Sinapis Semen
decocting containers
specific chromatograms
sinapine thiocyanate
similarity
decoction
