摘要
目的:对斑叶兰属植物斑叶兰Goodyera schlechtendaliana的化学成分进行分离纯化,从而丰富斑叶兰的化学成分,为斑叶兰后续深入研究奠定基础。方法:取斑叶兰干燥全草10 kg,85℃下用95%乙醇回流提取4次,合并提取液,减压浓缩得到粗浸膏,再将粗浸膏混悬于蒸馏水中,依次用等体积的石油醚、乙酸乙酯、正丁醇萃取,得到石油醚、乙酸乙酯和正丁醇萃取部位。采用硅胶,Sephadex LH-20,ODS等多种色谱填料和半制备高效液相对斑叶兰的乙酸乙酯和正丁醇萃取部位进行分离和纯化,利用1H-NMR,13C-NMR,MS等各种现代波谱技术以及化合物的理化性质并结合参考文献确定化合物的化学结构。结果:从斑叶兰乙酸乙酯部位和正丁醇部位分离得到10个化合物,分别是2,5-二羟基-4-甲氧基-9,10-二氢菲(1),间苯三酚三甲醚(2),3,4-二羟基苯甲酸(3),4-羟基苯甲醛(4),4-羟基苯甲酸(5),槲皮素(6),香兰素(7),胡萝卜苷(8),腺嘌呤(9),腺嘌呤核苷(10)。结论:化合物1~10均为首次从斑叶兰属植物中分离得到。
Objective: To isolate and purify the chemical constituents from Goodyera schlechtendaliana,and lay a foundation for further research on the effective materials of the plant. Method: The dried grass of G. schlechtendaliana( 10 kg) was heated by 95% EtOH for reflux extraction for 4 times at 85 ℃. The extract liquids were combined,decompressed and concentrated to obtain the crude extract. The crude extract was then suspended in distilled water and extracted with petroleum ether, EtOAc and n-BuOH in turn to obtain corresponding fractions. Silica gel, Sephadex LH-20, ODS and semi-preparation high performance liquid chromatography( HPLC) were used for the separation and purification of EtOAc and n-BuOH fractions. The structures of compounds obtained were identified by1 H-NMR,13 C-NMR,MS,physicochemical properties and reference literature. Result: Ten compounds were isolated and identified as 2,5-dihydroxy-4-methoxy-9,10-dihydroxyphenanthrene( 1),1,3,5-trimethoxybenzene( 2),protocatechuic acid( 3),p-hydroxybenzaldehyde( 4),p-hydroxybenzoic acid( 5),quercetin( 6),vanillin( 7),daucosterol( 8),adenine( 9) and adenosine( 10). Conclusion: Compounds 1-10 were obtained from the genus of Goodyera for the first time.
作者
殷启蒙
王润坤
胡琛
唐淳
赵之琛
刘量
YIN Qi-meng;WANG Run-kun;HU Chen;TANG Chun;ZHAO Zhi-chen;LIU Liang(Medical College of Yangzhou University,Yangzhou 225001,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2019年第2期181-185,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
江苏省中医药管理局科技项目(YB2015182)
扬州大学交叉学科建设项目(JCXK201520)
扬州大学大学生学术科技创新基金项目(x20180734,x20180738)
关键词
斑叶兰
化学成分
分离纯化
结构鉴定
Goodyera schlechtendaliana
chemical constituent
isolation and purification
structure identification
