摘要
目的建立天麻饮片UPLC指纹图谱和同时测定天麻饮片中天麻素、对羟基苯甲醇、巴利森苷B、巴利森苷C、巴利森苷A的超高效液相色谱分析方法。方法采用Waters Acquity超高效液相系统,Waters ACQUITY UPLC BEH C18(50 mm×2.1 mm,1.7μm)色谱柱,流动相为乙腈-0.1%甲酸水,梯度洗脱,检测波长270 nm(指纹图谱)、220 nm(含量测定),体积流量为0.3 mL/min,柱温35℃。通过相似度评价、主成分分析、偏最小二乘判别分析对28批天麻饮片指纹图谱进行分析,通过对照品比对对主要共有峰进行指认和含量测定。结果建立了不同产地天麻的指纹图谱,标定了20个共有峰,并指认了其中6个共有峰:天麻素(1号峰)、对羟基苯甲醇(3号峰)、对羟基苯甲醛(7号峰)、巴利森苷B(14号峰)、巴利森苷C(15号峰)、巴利森苷A(18号峰)。28批样品相似度均在0.90以上,主成分分析将不同产地天麻分为5组,并发现对羟基苯甲醇、对羟基苯甲醛、巴利森苷A等6个差异成分。同一成分在各产地天麻饮片中的含量差异明显,不同成分的波动范围不同,各产地5种成分总量贵州(2.52%)>云南(2.49%)>陕西(2.33%)>湖北(2.10%)>浙江(1.90%)>安徽(1.65%)。结论建立不同产地天麻UPLC指纹图谱结合多成分含量测定方法为完善天麻饮片的质量控制提供了参考。
Objective To establish the analysis method of UPLC fingerprint and simultaneous determination of five active components(gastrodin, 4-hydroxybenzyl alcohol, parishin B, parishin C, and parishin A). Methods The analysis was performed on a Waters Acquity with a Waters ACQUITY UPLC BEH C18(50 mm × 2.1 mm, 1.7 μm) column. The gradient mobile phase was acetonitrile and 0.1% formic acid-water solution, flow rate was set at 0.3 mL/min, the injection volume was 0.2 μL and the temperature of column was 35 ℃. The detection wavelengths were set at 270 nm(fingerprint), 220 nm(content determination). The 28 batches of samples fingerprint were analyzed by similarity evaluation, principal component analysis(PCA), and partial least squares discriminant analysis(PLS-DA). The identification and content determination of the main common peaks were carried out by comparison with reference. Results We set up the common mode of the fingerprint with 20 common peaks, and six of them were identified, which were gastrodin(1), 4-hydroxybenzyl alcohol(3), p-hydroxybenzaldehyde(7), parishin B(14), parishin C(15), and parishin A(18). The similarities among 28 samples were all above 0.90. PCA was used to divide the samples into five groups, and six different substances were found. The content of the same component and the fluctuation range among different components were all different. The content of the five components of each origin was Guizhou 2.52% > Yunnan 2.49% > Shaanxi 2.33% > Hubei 2.10% > Zhejiang 1.90% > Anhui 1.65%. Conclusion The establishment of UPLC fingerprint and simultaneous determination of five active components provide a reference for quality control of Gastrodia Rhizoma.
作者
李平
郝敏
苏联麟
季德
宋嬿
秦思睿
陆兔林
毛春芹
LI Ping;HAO Min;SU Lian-lin;JI De;SONG Yan;QIN Si-rui;LU Tu-lin;MAO Chun-qin(Nanjing University of Chinese Medicine,Nanjing 210023,China;Shanghai Huayu Pharmaceutical Co.,Ltd,Shanghai 200002,China;Wuhan University,Wuhan 430000,China)
出处
《中草药》
CAS
CSCD
北大核心
2018年第23期5665-5671,共7页
Chinese Traditional and Herbal Drugs
基金
国家中药标准化项目"天麻等6种中药饮片标准化建设"(ZYY-2017-137)
