摘要
目的观察清宫寿桃丸对利血平、亚硝酸钠、乙醇诱导的记忆障碍小鼠学习记忆的影响。方法 ICR小鼠随机分为正常组、模型组、清宫寿桃丸(低、中、高剂量)组、金纳多组,每组14只。正常组和模型组给予等量的蒸馏水,清宫寿桃丸低、中、高剂量组分别按0.9、1.8、3.6 g/kg给药,金纳多组按0.12 g/kg给药,给药15天。干预14天后,获得性记忆障碍模型、巩固性记忆障碍模型、再现性记忆障碍模型分别使用利血平、亚硝酸钠及乙醇造模。干预15天后采用跳台实验及避暗实验评估学习记忆能力。结果与正常组比较,三种模型小鼠错误次数均明显增多,潜伏期均明显缩短(P<0.05);与模型组比较,清宫寿桃丸不同剂量均可减少三种模型小鼠的错误次数(P<0.05),其中高剂量还可延长获得性记忆障碍小鼠的潜伏期(P<0.05);另外金纳多可减少巩固性记忆障碍小鼠的错误次数(P<0.05)。结论清宫寿桃丸可改善获得性记忆障碍、巩固性记忆障碍和再现性记忆障碍,在改善记忆障碍方面的应用范围较广。
Objective To observe the effect of Qinggong Shoutao Pill on the dysmnesia models induced by reserpine, sodium nitrite and ethyl alcohol. Methods ICR mice were randomly divided into normal group, model group, Qinggong Shoutao Pill group(low, medium and high dose), and ginaton group, with 14 mice in each group. The normal and model groups were given equal amounts of distilled water, the low, medium and high dose of Qinggong Shoutao Pill groups were respectively given at the dose of 0.9 g/kg, 1.8 g/kg and 3.6 g/kg, and ginaton group was given at the dose of 0.12 g/kg. After drug treatment for 14 days, the dysmnesia models of memory acquisition, consolidation and reappearance were established by reserpine, sodium nitrite and ethyl alcohol respectively. The memory results were estimated by using Step-down Test and Dark-avoiding Test after 15-days-intervention. Results Compared with the normal group, the number of errors in three dysmnesia models was significantly increased, and the latent time was significantly shortened(P<0.05). Compared with the model groups, different dose of Qinggong Shoutao Pill could significantly reduce the number of errors(P<0.05), and the high dose of Qinggong Shoutao Pill could prolong the latent time in mice with acquired dysmnesia(P<0.05). In addition, ginaton could reduce the number of errors in mice with consolidated dysmnesia(P<0.05). Conclusion Qinggong Shoutao Pill could improve the memory acquirement, consolidation and reappearance, and has a more widely application in the treatment of dysmnesia.
作者
袁蓉
张业昊
王燕
丛伟红
陈可冀
YUAN Rong;ZHANG Ye-hao;WANG Yan;CONG Wei-hong;CHEN Ke-ji(Cardiovascular Laboratory,Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing (100091);Graduate School, Beijing University of Chinese Medicine,Beijing (100029);Integrated Traditional and Western Medicine Center for Cardiovascular Disease,China-Japan Friendship Hospital,Beijing (100029))
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2019年第1期88-91,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金项目(No.81373821)
归国留学择优资助项目
中国博士后科学基金项目(No.2014M550134)
关键词
清宫寿桃丸
记忆障碍
学习记忆
老年性痴呆
Qinggong Shoutao Pill
amnesia
memory and learning
Alzheimer′s disease
