摘要
以伽师瓜和86-1甜瓜为试材,损伤接种浓度为1×106 CFU/mL链格孢孢子悬浮液20μL,置于6～8℃冷库贮藏,测定果实苯丙烷代谢相关酶和产物含量,研究两种甜瓜抗链格孢侵染过程中苯丙烷代谢相关酶和产物积累能力的差异,探讨伽师瓜耐藏机制。结果表明:损伤接种Alternaria alternata后,伽师瓜果实的发病率及病斑大小均低于86-1甜瓜;贮藏前期,甜瓜果实中苯丙氨酸解氨酶(PAL)、肉桂酸-4-羟化酶(C4H)、4-香豆酰辅酶A连接酶(4CL)活性及总酚、类黄酮的含量迅速升高,表明苯丙烷代谢在A.alternata与甜瓜果实互作的早期发挥了积极的作用,贮藏后期,苯丙烷代谢的抗病作用显著降低;链格孢侵染处理诱导了甜瓜果实PAL、C4H、4CL等酶活性的升高,增加了总酚和类黄酮等植保素的生成含量,说明苯丙烷代谢对甜瓜果实黑斑病有重要作用,伽师瓜果实PAL、4CL和C4H活性以及总酚、类黄酮含量高于86-1甜瓜果实,说明伽师瓜苯丙烷代谢活性高于86-1甜瓜,伽师瓜具有更强的抵御抗链格孢侵染的能力。
In order to study the differences in the phenylpropanoid pathway-related enzymes and product accumulation ability between two melon cultivars against A.alternata infection and to explore the storage tolerance mechanism of Jiashi melon, Jiashi melon and 86-1 melon were inoculated with 20 μL A.alternata spore suspension(1×10^6 CFU/mL)and then stored at 6~8 ℃ to determine enzymes related to phenylpropanoid pathway and product contents. The results showed that, the incidence of disease and lesion diameter of the inoculated Jiashi melon fruit were lower than that of 86-1 melon. At the early stage of storage, the activities of phenylalanine ammonia-lyase(PAL), cinnamate4-hydroxylase(C4 H), 4-coumaryl coenzyme A ligase(4 CL) and the contents of total phenol and flavonoid increased rapidly in muskmelon fruit, which suggested that phenylpropanoid pathway played a positive role in the interaction of A.alternata and muskmelon at early stages, however, the resistance role of phenylpropanoid metabolism decreased significantly in late storage stage. Alternaria infection treatment induced the increase of activities of PAL, C4 H, 4 CL and other enzymes in muskmelon fruit, and increased the content of phytochemicals such as total phenol and flavonoid, which suggested that phenylpropanoid pathway played an important role in melon black spot disease.However, both the activities of phenylpropanoid pathway-related enzymes and the phytochemicals contents of Jiashi melon were significantly higher than 86-1 melon, which indicated that Jiashi melon fruit had higher metabolic activity of phenylpropane and stronger resistance to A.alternata infection.
作者
张培岭
白羽嘉
马玲
王玉红
冯作山
ZHANG Pei-ling;BAI Yu-jia;MA Ling;WANG Yu-hong;FENG Zuo-shan(College of Food Science and Pharmacy,Xinjiang Agricultural University,Urumqi 830052,China)
出处
《保鲜与加工》
CAS
北大核心
2018年第6期1-6,12,共7页
Storage and Process
基金
国家自然科学基金项目(31460413)
关键词
甜瓜
链格孢
苯丙烷代谢
muskmelon
A.alternata
phenylpropanoid metabolism
