摘要
目的探讨祖师麻肠溶制剂对佐剂性关节炎大鼠的抗炎与镇痛作用。方法右后肢足跖皮内注射弗氏完全佐剂制备佐剂性关节炎大鼠模型。将大鼠分为对照组、模型组、泼尼松组,及中药低、中、高剂量组,每组12只。泼尼松组灌胃以泼尼松3.2 mg/kg,中药低、中、高剂量组分别灌胃以祖师麻肠溶制剂0.396、0.792、1.584 g/kg,对照组、模型组灌胃以等体积的7.5%CMC-Na溶液。各组均连续干预12 d,于造模后第7 d、第12 d计算大鼠足跖肿胀率、50%机械缩足反射阈值(MWT)、热缩足反射阈值(TWT);末次给药24 h后ELISA法测定大鼠血清白介素-6(IL-6)、前列腺素E2(PGE2)、肿瘤坏死因子-α(TNF-α)水平,HE染色观察大鼠膝关节和踝关节病理学改变。结果 (1)与对照组比较,模型组大鼠足跖肿胀率升高(P<0.01),50%MWT、TWT降低(P<0.01),血清IL-6、PGE_2、TNF-α水平升高(P<0.01)。(2)与模型组比较,泼尼松组和中药低、中、高剂量组大鼠足跖肿胀率降低(P<0.01),50%MWT、TWT升高(P<0.01),血清IL-6、PGE_2、TNF-α水平降低(P<0.01)。(3)泼尼松组与中药各剂量组比较、中药各剂量组组间比较,上述指标差异无统计学意义(P>0.05)。结论祖师麻肠溶制剂具有明显的抗炎、镇痛作用,可明显改善佐剂性关节炎大鼠膝关节及踝关节病理损伤。
Objective To investigate the anti-inflammatory and analgesic effects of Daphnis Cortex enteric-coated preparation on adjuvant arthritis rats.Methods The rat model of adjuvant arthritis was established by intradermal injection of Freund’s complete adjuvant. The rats were divided into the control group,model group,prednisone group,and low,middle and high dose groups of Chinese medicine,12 rats in each group. The prednisone group was given prednisone 3.2 mg/kg by gavage,and the low,middle and high dose groups of Chinese medicine were given the medicine 0.396 g/kg,0.792 g/kg,1.584 g/kg,respectively by gavage. The control group and the model group were given the same volume of 7.5% CMC-Na solution. After continuous intervention for 12 days,the swelling rate of voix pedis,the 50% mechanical withdrawal threshold( MWT) and thermal withdrawal threshold( TWT)were calculated on the 7 th and 12 th day after modeling. The serum levels of interleukin-6( IL-6),prostaglandin E2( PGE2) and tumor necrosis factor-α( TNF-α) were measured by ELISA 24 hours after the last administration. The pathological changes were observed by HE staining in the knee and ankle joints of the rats. Results(1)Compared with the control group,the swelling rate of voix pedis in the model group was higher than that in the model group( P<0.01) and the value of 50%MWT,TWT was decreased( P<0.01) but the levels of IL-6,PGE2,TNF-α in serum were decreased( P<0.01).(2) Compared with the model group,the swelling rate of voix pedis was decreased( P<0.01),the 50% MWT and TWT were increased( P<0.01),and the levels of the serum IL-6,PGE2,TNF-α were decreased in the prednisone group,and in the low,middle and high dose groups of Chinese medicine( P<0.01).(3) There was no significant difference among prednisone group and Chinese medicine groups in the above indicators( P>0.05).Conclusion Daphne Cortex enteric-coated preparation has obvious anti-inflammatory and analgesic effects. It can significantly improve the pathological damage of knee joint and ankle joint in rats with adjuvant arthritis.
作者
程文豪
张天睿
李颖慧
马丹
宋敬怡
张仲毅
张硕峰
CHENG Wenhao;ZHANG Tianrui;LI Yinghui;MA Dan;SONG Jingyi;ZHANG Zhongyi;ZHANG Shuofeng(College of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China;Shanhaiguan Pharmaceutical Co.,Ltd.,Qinhuangdao 066000,China)
出处
《上海中医药杂志》
2019年第1期101-105,共5页
Shanghai Journal of Traditional Chinese Medicine
基金
中国医药教育协会孙思邈中医药研究专项课题(2016SKT-M006)
关键词
祖师麻
佐剂性关节炎
大鼠
抗炎
镇痛
Daphnes Cortex
adjuvant arthritis
rat
anti-inflammatory
analgesic
