摘要
液态凉茶采用甲醇-乙腈(1:1,V/V)超声提取,经过简单的稀释和过滤,经C_(18)色谱柱分离,以甲醇-0. 5%甲酸水溶液为流动相进行梯度洗脱,电喷雾正离子模式电离,多反应监测模式检测,标准曲线法定量。19种药物在10～200μg/L线性关系良好,相关系数均大于0. 9963,加标浓度为20,50,100μg/L,平均回收率在69. 6%～117. 8%,相对标准偏差(RSD)在0. 92%～13%,检出限(LOD)在0. 2～5. 0μg/L。方法适用于凉茶等植物型饮料中非法添加化学药物的快速测定。
A high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method was developed for simultaneous determination of 19 kinds of illegally added chemical substances in herbal tea.The liquid herbal tea was extracted by methanol aeetonitrile (1:1,V/V).After simple dilutioia and filtration,it was separated on C18 column with the mibile phase of aqueous solution of methanol -0.5%formic acid was used as the mobile phase for gradient elution.The determination was carried out on mass spectrometer with electrosprayion source in the positive mode and monitored by the multiple reaction monitoring (MRM)mode.The samples were quantified by the matrixmatched calibration curves.The 19 drugs showed good linearity in the range of 10 -200μg/L with the correlation coefficients (r)higher than 0.9963 at the spiked levels of 20,50,100μg/L.The average recoveries were in the range of 69.6%-117.8% with the relative standard deviations in the range of 0.92%-13%.The limits of detection of the method were between 0.2 and 5.0g/L.The method is suitable for the rapid determination of illegal chemical drugs in herbal beverages such as herbal tea.
作者
胡佳哲
赖宇红
HU Jia-zhe;LAI Yu-hong(Guangdong Institute for Drug Control,Guangzhou 510180)
出处
《分析试验室》
CAS
CSCD
北大核心
2019年第2期230-234,共5页
Chinese Journal of Analysis Laboratory
关键词
凉茶
非法添加
化学药物
高效液相色谱-串联质谱
Herbal tea
Illegal addition
Chemical drugs
High performance liquid chromatography- tandem mass spectrometry
