摘要
硫酸奎宁作为荧光试剂,最大激发波长为350 nm,最大发射峰位于450 nm处。硫酸奎宁与不同浓度的Fe^(3+)混合后,发生荧光猝灭,lg(I0/I)与c(Fe^(3+))呈良好的线性关系,基于此建立了硫酸奎宁荧光探针检测Fe^(3+)的新方法。研究表明,该方法检测Fe^(3+)浓度的线性范围为0. 7～400μmol/L,相关系数为R=0. 999 5,检测限为140 nmol/L。在实际样品分析中,当分别加入4、10和70μmol/L Fe^(3+)时,回收率为99. 4%～100. 4%,相对标准偏差(RSD)为0. 8%～2. 1%。该方法操作简便、反应速度快、灵敏度高,能用于实际样品中Fe^(3+)的检测分析。
As a fluorescent reagent,quinine sulfate has a maximum excitation wavelength of 350 nm and a maximum emission peak of 450 nm.When quinine sulfate was mixed with different concentrations of Fe^3+,the fluorescence of quinine sulfate was quenched.It was found that lg( I0/I) showed a good linear relationship with c( Fe^3+).Based on this,a new method was developed for the detection of Fe^3+by quinine sulfate as a fluorescent probe.The linear range of Fe3+concentration was 0. 7 ~ 400 μmol/L with a correlation coefficient of 0. 999 5.The limit of detection was140 nmol/L.When 4,10,70 μmol/L Fe^3+were added to the real samples,the recovery range was 99. 4% ~ 100. 4% and the relative standard deviation( RSD) range was 0. 8% ~ 2. 1%. This method was simple,rapid and sensitive and can be used for the detection of Fe^3+in real samples.
作者
李敏
敬小莉
谈文林
LI Min;JING Xiao-li;TAN Wen-lin(Department of Basic Education,Dazhou Vocational and Technical College,Dazhou 635000,China;Department of Chemistry and Chemical Engineering,Sichuan University of Arts and Science,Dazhou 635000,China)
出处
《化学试剂》
CAS
北大核心
2019年第2期173-176,共4页
Chemical Reagents
基金
达州市科技局应用基础研究项目(JCY16120)
四川省科技厅应用基础项目(2015JY0033)
四川省教育厅重点项目(18ZA0414)
