摘要
目的 检测肿瘤 睾丸抗原SSX1基因mRNA在肝细胞肝癌 (HCC)中的表达情况。方法 用逆转录 聚合酶链反应 (RT PCR)对 47例HCC患者的癌组织和相应癌旁组织以及 1 5例肝硬化和 1 5例正常肝组织的SSX1基因mRNA表达情况进行检测 ,随机选择 4例RT PCR扩增产物的目的片段进行DNA序列测定。结果 47例HCC患者中 ,39例表达SSX1mRNA ,阳性率为83 % ,相应的癌旁组织中有 3例为弱阳性 ,其中 2例患者获得了离癌灶更远处的活检组织 ,对其进行RT PCR检测 ,结果显示阴性 ;所测的 1 5例肝硬化和 1 5例正常肝组织均未检测到SSX1的表达。4例DNA测序结果表明RT PCR产物确为SSX1cDNA。SSX1的表达与年龄、性别、肿瘤大小、分化程度、血清甲胎蛋白 (AFP)水平、乙型肝炎病毒 (HBV)和丙型肝炎病毒 (HCV)感染无显著相关性(P >0 .0 5)。结论 SSX1在HCC中呈高比例、高特异表达 。
Objective To investigate the expression of cancer testis antigen SSX1 gene mRNA in hepatocellular carcinoma.Methods The expression of SSX1 mRNA was detected using reverse transcription polymerase chain reaction (RT PCR) in hepatocellular carcinoma(HCC) tissues and the corresponding adjacent non HCC tissues from 47 HCC patients,15 samples of cirrhosis and 15 samples of normal liver tissues.Four samples selected randomly from SSX1 PCR positive products were sequenced.Results SSX1 mRNA was detectable in 39/47 (83.0%) of HCC samples and 3/47 (6.4%) of the corresponding adjacent non HCC tissue,respectively.Of the latter 3 patients,biopsy samples far from tumor were obtained in 2 patients and RT PCR indicated no expression of SSX1 mRNA detectable in these two samples.In addition,None of 15 samples of cirrhosis and 15 samples of normal liver tissue expressed SSX1 mRNA.The DNA sequence confirmed that the RT PCR products were truly SSX1 cDNA.No relationship was found between the expression of SSX1 and clinical indicators such as age,sex,tumor size,degree of tumor differentiation,serum α fetoprotein level and infection of hepatitis B virus or hepatitis C virus (P>0.05).Conclusion SSX1 mRNA is expressed with a high percentage and specificity in hepatocellular carcinomas.The SSX1 gene product is potential target for antigen specific immunotherapy of HCC.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2002年第2期110-111,共2页
Chinese Journal of Experimental Surgery
