摘要
研究了不同的油脂提取工艺对产油酵母油脂产率的影响。以高产油酵母JM-D为原料,以油脂产率为测定指标,比较了蜗牛酶法、超声波破碎法、反复冻融法、细胞匀浆机破碎法、微波破碎法、研磨法以及酸热法对酵母细胞壁的破碎效果,并对有机溶剂提取法与索氏抽提法提取细胞内油脂的效果进行比较。结果显示,蜗牛酶法的细胞破碎效果最好,油脂产率高达46.2%;其次是反复冻融(100℃水浴解冻)法,油脂产率高达43.4%;研磨法+酸热法的油脂提取效果也较好,油脂产率达到37.8%。研磨后,采用索氏抽提法的提取效果优于有机溶剂提取法的。
The effects of different oil extraction processes on oil productivity of oil-producing yeast were studied. With high oil-producing yeast strains JM-D as raw material and oil productivity as indicator, the crushing effects of JM-D cell wall by different methods （ snail enzymatic method,ultrasonic crushing method,repeated freeze-thaw method, cell homogenizer crushing method, microwave crushing method, grinding method and acid-hot method） were compared,and the extraction effects of oil from JM-D cell by organic solvent extraction method and Soxhlet extraction method were also compared. The results showed that the crushing effect of JM-D cell wall by snail enzymatic method was the best,the oil produc-tivity reaching 46. 2%,followed by repeated freeze-thaw method（thawing in 100℃water bath） and acid-hot method combined with grinding method,and their oil productivities reached 43. 4% and 37. 8% respec-tively . The extraction effect of oil from JM-D cell after grinding by Soxhlet extraction method was superior to that by organic solvent extraction method.
出处
《中国油脂》
CAS
CSCD
北大核心
2014年第7期13-16,共4页
China Oils and Fats
基金
河南省教育厅科技攻关项目(2011B180043)
河南省教育厅科学技术研究重点项目(13A180830)
河南省科技厅科技攻关重点项目(132102310039)
关键词
细胞破碎
油脂提取
酸热法
索氏抽提
产油酵母JM-D
oil-producing yeast JM-D
cell crushing
oil extraction
acid-hot method
Soxhlet extraction
