摘要
目的:研究黄芪多糖对肝纤维化小鼠骨丢失的防治作用。方法:40％CCl_4花生油皮下注射5W致小鼠肝纤维化,用黄芪多糖防治6W后,观察与肝损伤相关的各项生化指标和肝脏病理切片结果,测定小鼠右股骨的中Ca^(2+)和其他微量元素以及骨羟脯氨酸(Hyp)的含量。结果:单用CCl_4小鼠呈现典型的慢性肝损伤后肝纤维化的改变,骨重量和骨钙总量及骨羟脯氨酸的含量明显减少(P<0.01),而黄芪多糖治疗组有明显的护肝及对抗骨丢失作用,其作用优于秋水仙碱治疗组。结论:黄芪多糖在所用的剂量下对小鼠肝纤维化及骨丢失有一定预防作用。
Objective: To study the relationship between hepatic fibrosis and osteoporosis, and to observe the effects of Astragalus polysaccharides on preventing bone loss. Methods: Forty PCR Mice were randomly divided into 4 groups. Group A mice were controls: Group B mice were given SC injection of 40% CC14 10ml/kg once per 5 days fibrosis model group: Group C were given Colchicine 0. 1 mg/kg orally in addition to CCl4 SC. Group D were given Astragalus polysaccharides 10 mg/kg orally in addition to CCU SC. The four groups of mice were treated for 42 d. The liver injury indexes were measured and the mineral elements and Hydroxyproline of femur were determined. Results: Compared with group A, the serum enzymes of alanine aminotransferase (ALT), aspartic acid aminotransferase (AST) were markedly increased but serum albumin (Alb) and A/G were decreased distinctly in group B whose liver slides also showed typical liver cirrhosis. The study of the bone showed the dried weight of femur was markedly reduced and the bone Calcium content and bone Hydroxyproline content were also significantly decreased in group B. On the other hand, the result also demonstrated bone copper and bone magnesium were increased in group B. However,in group D and group C,AP inhibited markedly the decrease of the Serum enzymes and increase Calcium content and Hydroxyproline content of the bone than group B, It seen that the effect of group D could be better than group C. so that the bone mass loss was prevented effectively, by Astragalus polysaccharides. Conclusion: The bone mass was lost in mice with chronic hepatic injury induced by CC14 and Astragalus polysaccharides showed protection of liver tissue and preventive effect on bone loss in such
出处
《中西医结合肝病杂志》
CAS
2002年第2期95-98,共4页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
基金
广东省卫生厅资助课题
