摘要
目的探讨大鼠局灶性脑缺血再灌注后各时相的白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的表达及中药川芎嗪对其表达的影响。方法采用放免法测定IL-6、TNF-α的动态变化。结果对照组IL-6表达在12h时显著增高(P<0.05);实验组在再灌注6h时表达显著增高(P<0.05),12h时仍显著增高(P<0.05)。对照组TNF-α缺血早期表达即增高,再灌注12h时最显著,而实验组明显降低(P<0.05)。结论TNF-α加重脑缺血再灌注损伤;IL-6可能作为一种脑缺血后神经细胞死亡的内生拮抗剂,起到神经保护作用;川芎嗪可抑制脑缺血再灌注后TNF-α的表达,使IL-6的表达提前,可能是川芎嗪对抗脑缺血再灌注损伤的机制之一。
Objecte To investigate the effect of ligustrazine on IL 6 and TNF α expression after local cerebral ischemia reperfusion. Methods IL 6 and TNF-α expression were detected by radioimmunoassay.Results At 12 h after reperfusion higher IL 6 expression in control group was found(P< 0.05); In experimental group the IL 6 expression was obviously increased at 6 h and 12 h compared with the control group (P< 0.05). TNF-αincrease in control group was investigated in early ischemic stage, reaching the maximum at 12 h after reperfusion. The level of TNF-αin experimental group significantly was lower than control animals.Conclusion The cerebral ischemia reperfusion injury was deteriorated by TNF α, while the effect of neuron protection own to the benefit of IL-6. Ligustrazine inhibit the expression of TNF α and increase expression of IL 6 early, which indicate ligustrazine may playing an important role in neuro-protection.
出处
《中国临床康复》
CSCD
2002年第7期968-969,共2页
Chinese Journal of Clinical Rehabilitation
