摘要
以 6个早实核桃 (JuglansregiaL .)新品种为试材 ,对长期继代培养的试管嫩茎的生根进行了研究 ,结果表明 :诱导方法、外源IBA的水平、嫩茎发育状态以及光照条件等对生根具有明显的影响。选取生长旺盛、节间较长、叶较小呈嫩黄绿色的幼态嫩茎 ,采用 2步诱导生根法 ,即黑暗条件下 ,在IBA为 5 0～ 10 0mg·L- 1 的 1 4DKW培养基中诱导 10～ 15d ,而后转移至不含IBA的DKW培养基中 ,利用这一方法顺利地诱导了嫩茎生根 ,在对 6个品种的应用中获得了 6 0 5 %～ 89 7%的生根率 ,平均根数 2 0～ 3 6。生根的嫩茎已成功地进行了下地移栽。同时就根原基诱导过程中嫩茎内源IAA和ABA水平变化进行了研究 。
Measures to promote rooting of in vitro shoots were conducted with six precocious walnut cultivars which had been subcultured for 4 years. It was found that successful rooting was closely related to inducing method, duration of IBA treatments, IBA concentrations, light conditions, and developing state of the shoots. The rooting rates of 60 5%～89 7% and 2 0～3 6 roots per shoot were achieved in six cultivars by adopting tender shoot with smaller leaves and longer internodes and using the two steps method that was firstly induced on 1/4 DKW medium with IBA between 5 0 to 10 0 mg·L -1 for 10～15 days under dark condition, then transferred to the DKW medium without IBA under light. Meanwhile, changes of IAA and ABA level in shoots were monitored during rooting inducing. The regulations and control of hormones during walnut shoot rooting was discussed in this paper. The rooted shoots have been successfully transplanted to field.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2002年第2期32-37,T001,共7页
Scientia Silvae Sinicae
基金
国家高技术应用部门发展项目 (2 0 0 0 2 6)"西部经济林良种壮苗技术研究"。
关键词
核桃
品种
试管嫩茎
生根
诱导
嫩茎发育状态
内源激素
Juglans regia L. cultivar, Shoot in vitro, Root promoting, Shoot developmental state, Endogenous hormone
