摘要
目的 了解在实验室条件下钩端螺旋体的生长规律 ,为进一步开展蛋白质组和基因表达谱的研究做准备。方法 使用pH为 7 4的EMJH液体培养基 ,在 30℃条件下置摇床上以 90r/min对该菌进行通气振荡培养 ,记录不同时间点的活菌数和培养物的光密度。结果 研究发现 ,钩体的生长与其它细菌培养相似 ,也有静止期、对数生长期、稳定期和衰老期。在该培养条件下 ,钩端螺旋体的对数生长期为培养的第 12～ 5 4h。在对数生长期 ,钩端螺旋体复制一代所需的时间约为 11 35h。并且 ,钩端螺旋体活菌计数和光密度值OD60 0 之间有较好的相关性。结论 本文比较详细地描述了钩端螺旋体的生长参数—活菌数和OD60 0 ,并找到了钩端螺旋体生长的对数生长期 ,在严格控制试验条件的情况下 ,可以使用测定液体培养物的OD60 0值的方法来推算菌体数 。
Aim To explore the growth characteristics of leptospira for the purpose of performing the proteome analysis of the organism.Method Being one of the fastidious bacteria, Leptospira was incubated in EMJH medium using a shaker with a vibration frequency of 90 rpm.The pH of the medium was controlled at 7.4 and the temperature at 30℃.The viable count and OD600 of the leptospira over time was continually measured.Result We found that the growth curves of leptospira followed the same lag, logarithmic, stationary and aging phases as in other bacteria.The logarithmic phase was from 12 to 54 hours under the defined condition.A typical doubling time for lepospira under such an optimum condition was about 11.35 hours (measured by viable count).Correlation analysis showed that viable count and OD600 had a very good correlation.Conclusion We described in detail the two measurements, viable count and OD600, calculated the logarithmic phase and a typical doubling time under defined conditions, and found that OD600 was an optimum measurement substitute for viable count to reflect the growth of leptospira.
出处
《中国人兽共患病杂志》
CSCD
北大核心
2002年第3期60-63,共4页
Chinese Journal of Zoonoses
