摘要
目的 :建立测定黄疸茵陈颗粒中 3种蒽醌成分大黄素、大黄酸、大黄酚含量的方法。方法 :采用HPLC法 ,使用C1 8柱 ,流动相为甲醇 - 1 %高氯酸 (80∶2 0 ) ,检测波长为 2 80nm。结果 :此方法能使样品中的 3种蒽醌类成分得到良好分离且线性关系良好 ,平均加样回收率为 :大黄酸 97.43 % ,RSD =1 .90 % ;大黄素 98.45 % ,RSD =1 .62 % ,大黄酚 98.87% ,RSD =1 .50 %。结论 :本法简便、快速 ,结果令人满意 ,可用于作为黄疸茵陈颗粒质量控制方法之一。
Objective:To develop a method to determine the contents of emodin, rhien and chrysophanol in Huangdanyinchen Granules. Methods: HPLC was used to determine the contents of emodin, rhien and chrysophanol in Huangdanyinchen Granules. The separation was performed on YWG ODS column with methol perchloric acid (1%) (8∶2) as a mobile phase and the wavelength of UV detector was 280nm.Results: The separating degree and the linearity of this method was well with the average recovery of rhien: 97.43%, RSD= 1.90%; Emodin:98.45%,RSD=1.62%;Chrysophanol: 98.87%, RSD=1.50%, respectively.Conclusions:The method is simple and rapid and gives satisfactory results. It can be suitable for quality controll of Huangdanyinchen Granules.
出处
《中成药》
CAS
CSCD
北大核心
2002年第5期340-341,共2页
Chinese Traditional Patent Medicine
