摘要
采用SDS PAGE研究了 10 8头麦洼牦牛和 98头九龙牦牛乳MUC1的生化遗传特性 ,结果表明 :牦牛乳MUC1呈现出多态性 ,表现为一条或两条迁移率不同的区带。SDS PAGE分析发现 4种分子量的MUC1区带 ,依次为A :2 0 8kd ,B :2 0 0kd ,C :196kd ,D :185kd ,分子量大于荷斯坦牛。麦洼牦牛乳MUC1基因座受A、B、C、D 4个复等位基因支配 ,有 9种基因型 ;九龙牦牛乳MUC1基因座受A、C、D 3个复等位基因支配 ,显示 5种基因型。两品种牦牛乳MUC1基因型分布差异极显著 (P <0 0 1) ,并且均偏离哈代—温伯格定律 (P <0 0 1) 。
The epithelial mucin,MUC1,in individual milk samples from 108 Maiwa yaks and 98 Jiulong yaks was resolved on SDS PAGE.Polymorphism of MUC1 consisted of one or two bands showing variable mobility.Mobility indices established for these bands provided evidence of four polymorphic forms according to their molecular weight on SDS PAGE:A:208 kd,B:200 kd,C:196 kd,D:185 kd,most of which were higher than that reported in Holstein cows.Milk MUC1 locus of Maiwa yak was controlled by A,B,C,and D alleles,exhibited nine genotypes;while in Jiulong yak was controlled by A,C,and D alleles,exhibited five genotypes.Significant difference of milk MUC1 genotypes distribution,which was not in accordance with Hardy Weinberg balance in Maiwa yaks or Jiulong yaks,existed between the two yak breeds.The genetic variation of milk MUC1 locus of Maiwa yaks was higher than that of Jiulong yaks.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2002年第3期235-238,共4页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金 (3 9870 60 7)
四川省科委及农业部动物生理生化重点实验室资助
