摘要
目的 : 观察壳聚糖抑制脂肪吸收的效果和机制 ,为发展营养健康的快餐食品提供科学依据。方法 : 1 .体外消化实验 磷钼酸比色法 ;2 .体内实验 将小鼠 2 4 0只随机分为对照(A)、高脂 (B)、壳聚糖 (C)、高脂加壳聚糖 (D) 4组 ,各组饲料脂肪和壳聚糖含量 (% )为 A(1 0 ,0 ) ,B(6 0 ,0 ) ,C(8,2 0 ) ,D(3 4 .3 ,1 4.8) ,饲养 45 d。测定血脂各项指标和粪中脂肪排出量 ,观察体重增长和抗应激能力的变化。结果 : 1 .模拟体外消化 ,壳聚糖与胆汁酸作用 ,在比例为 1∶ 8时 ,壳聚糖可以较好的络合胆汁酸 ,干扰机体对脂肪的吸收 ,使摄入的脂肪排出体外。2 .冰水游泳 ,证明食用适量壳聚糖对体质有增强和改善作用。 3 .与高脂组 (B)相比 ,C和 D的血脂、体重均无显著增加 ,脂肪排出量则显著增加。
Objective: To research the effect and mechanism of how chitosan restrain fat-absorbtion, and provide references for developing a kind of nutritional and healthy fastfood. Methods: 1.In vitro digestion: Phosphomolybdic acid colorimetry; 2.In vivo experiment:240 rats were divided into four groups and fed with different diets: basal diet(Group A), high fat diet (Group B), chitosan supplemented diet (Group C), high fat and chitosan supplemented diet (Group D). The fat and chitosan content(%) of diet were A(10,0), B(60,0), C(8,20), D(34.3,14.8) respectively. Determine the blood lipids and fat output in dung, and observe the weight gain and swimming time. Results: 1.In vitro experiment showed chitosan acted on cholic acid at the best proportion 1∶8. 2.In vivo experiment showed chitosan can restrain fat-absorbtion, and increase fat output. Swimming in ice-water showed chitosan improved rats performance. Compared with group B, the blood lipids and weight of group C and D had no obvious change, but the fat-output much increased.
出处
《营养学报》
CAS
CSCD
北大核心
2002年第1期53-57,共5页
Acta Nutrimenta Sinica
基金
国家人事部留学归国人员科技活动资助项目( No.人 2 0 0 10 6 AD)
关键词
胆汁酸
血脂
营养学
壳聚糖
抑脂作用
chitosan
fat-absorbing inhibition
cholic acid
hyperlipemia
