摘要
目的 :探讨无毒棉籽水提物 (CTN W )抗抑郁、抗焦虑作用的可能机理。方法与结果 :提取大鼠大脑皮层突触膜并与CTN W 0 .0 1,0 .0 3,0 .10 ,0 .30mg·mL-1直接孵育 ,放免法测定腺苷酸环化酶 (AC)活性的改变 ,发现CTN W可剂量依赖地激活AC ;MTT比色法研究表明 ,CTN W 0 .0 8,0 .4 0 ,2 .0 0mg·mL-1与皮质酮 2× 10 -4mol·L-1共孵PC12细胞 4 8h后可防止皮质酮所致的PC12神经细胞损伤。结论 :CTN W的作用可能与信号转导系统AC cAMP通路的激活 ,从而对损伤神经元产生保护作用有关 ,此二者共同组成了CTN
Objective:To study the possible mechanism of aqueous extract of detoxified cottonseeds (CTN W). Method and result:CTN W 0.01,0.03,0.10,0.30 mg·mL -1 was incubated directly with the synaptic membrane extracted from the cerebral cortex in rats, and adenylyl cyclase (AC)activity was detected by using radio immunoassay. Result: Showed that CTN W could activate AC in a dose dependend manner.After incubation with PC12 cells in the presence of corticosterone 2×10 -4 mol·L -1 for 48h,CTN W 0.08,0.4,2 mg·mL -1 protected PC12 cells from the lesion induced by corticosterone. Conclusion:Antidepressant and anxiolytic effects of CTN W are related with the activation of AC cAMP pathway in signal transduction system, thus protecting neurons from the lesion.These two aspects maybe partly form the mechanism of CTN W's action.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2002年第6期442-446,共5页
China Journal of Chinese Materia Medica
关键词
无毒棉籽水提物
皮质酮
PC12细胞
损伤
对抗作用
aqueous extract of detoxified cottonseeds
adenylyl cyclase
corticosterone
PC12 cells
