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α-淀粉酶在短短芽孢杆菌中的分泌表达 被引量：4

Secretory expression of α-amylase in Brevibacillus brevis
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摘要 应用PCR技术从枯草杆菌168中分离出α-淀粉酶基因。将其引入分泌表达载体pBKE50后,用Tris-PEG法转入短短芽孢杆菌50中,发现α-淀粉酶以活性形式被分泌表达。酶测活分析表明α-淀粉酶活性约为出发菌枯草杆菌168的1.7倍。 Theα-amylase gene of Bacillus subtilis168was isolated by PCR and used to construct recombinant plasmid pBKE50/α-amy with vector pBKE50.Soluble and biologically activeα-amylase was secreted directly into the culture medium after pBKE50/α-amy had been introduced into Bacillus brevis50by the Tris-PEG method,and the amount of enzymes produced by Bacillus brevis50(pBKE50/α-amy)was1.7times larger than that produced by Bacillus subtilis168.
作者 彭清忠 张惟材 朱厚础
机构地区 军事医学科学院生物工程研究所
出处 《生物技术通讯》 CAS 2002年第3期167-169,共3页 Letters in Biotechnology
关键词 Α-淀粉酶 短短芽孢杆菌 分泌 表达 Brevibacillus brevis α-amylase secretory expression
分类号 Q936 [生物学—微生物学]
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参考文献6

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共引文献2

同被引文献31

  • 1沈卫锋,牛宝龙,翁宏飚,何丽华,孟智启.枯草芽孢杆菌作为外源基因表达系统的研究进展[J].浙江农业学报,2005,17(4):234-238. 被引量：27
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引证文献4

二级引证文献15

生物技术通讯
2002年 第3期

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