摘要
本实验用CaCl_2溶液对香蕉(Musa acuminata cf. 'Dwarf Davendish')组织进行真空浸透处理,研究Ca^(2+)对香蕉采后乙烯释放、EFE活性、ACC水平以及ACC/MACC比值的影响。结果表明,Ca^(2+)处理可抑制香蕉果皮和果肉组织乙烯生成,对抑制果皮的乙烯生成尤为明显。Ca^(2+)处理还可降低内源ACC水平,抑制EFE活性。结果还显示,Ca^(2+)处理对组织中ACC/MACC比值有一定影响。
Banana, ( Musa acuminata cv. 'Dwarf Davendish' ) fruit tissues were vacuum infiltrated with CaCl2 solutions in order to determine the influence of calcium upon ethylene production, EFE activity, ACC content and ACC/MACC ratio. The results showed that calcium treatment inhibited ethylene production in both peel and pulp, particularly in peel. Ethylene production in peel treated with 3 concentrations of CaCl2 solutions (0.2, 0.6 and 0.8 mol/L) remained lower than that in the control throughout the period of 2-6 hours after treatment.Ethylene production in pulp infiltrated with 0.2 and 0.6 mol/L CaCl2 were inhibited during 2-3 hours after treatment,and ethylene production was reduced by 0.8mol/L CaCl2 treatment throughout 2-6 hours (Fig. 1 ) . Calcium infiltration (with 0,2, 0.4 and 1.0 mol/L CaCl2 ) lowered the internal ACC level in both peel and pulp (Tab. 1).EFE activity in peel and pulp treated with 0.05, 0.1, 0.2 and 0.5 mol/L CaCl2 were reduced (Tab. 2 ) . The result also showed that calcium treatment contributed to the change of ACC/MACC ratio (Fig. 2) .
The results suggest that the inhibition of Ca2+ on ethylene production in banana tissues, does not merely act on the step of ACC to ethylene, Ca2+ may also regulate both ACC biosyn thesis and the bioconversion of ACC to MACC.
基金
福建省自然科学基金资助课题的组成部分
关键词
香蕉
采收
乙稀
钙
释放
Banana,calcium, ethylene(C2H4), ethylene forming enzyme (EFE) , 1-aminocyclopropane-Icarboxylic acid (ACC ) , 1-malonyl-aminocyclopropane-1-carboxylic acid (MACC)
