摘要
利用设计合成的 1对根肿病菌 (Plasmodiophorabrassicae)特异性寡聚核苷酸引物 (前引物 :5’GAAGATGCCCACGCCGTCGT3’和后引物 :5’ATCTGTTCAGCAAAGCGTCGA3’) ,对分离自十字花科作物 (白菜、青菜、甘蓝、芥蓝、花椰菜等 )及土壤中的根肿病菌全基因组DNA进行PCR(PolymeraseChainReaction)特异性扩增试验。试验结果表明 ,试验设计合成的引物能从十字花科根肿病菌全基因组DNA中扩增到 6 2 9bp长度的分子片段 ,该对引物可用于十字花科蔬菜根肿病的分子鉴定和分子监测 ,为十字花科根肿病的早期准确诊断和病菌检测提供了快速、简便的方法。
Genomic DNA of Plasmodiophora brassicae extracted from cruciferae clubroot( Brassica pekinensis,Brassica chinensis,Brassica oleracea var. capitata L., Brassica caulorapa, Brassica alboglabra, Brassica oleracea var. botrytis L.)and soil was amplified with a pair of oligonucleotide specific primer designed according to the gneonic of Plasmodiophora brassicae . The results showed about 629?bp fragment was amplified with the pair of primers from genomic DNA of Plasmodiophora brassicae . The pair of primers will be useful for molecular identification and detection of cruciferae clubroot. The results provided a rapid and simple method to detection and forecast.
出处
《云南农业大学学报》
CAS
CSCD
2002年第2期137-139,157,共4页
Journal of Yunnan Agricultural University
基金
云南省"十五"科技攻关项目部分研究内容 (2 0 0 0A3- 0 2 )
关键词
十字花科
蔬菜
根肿病菌
PCR检测
菌株
引物
Plasmodiophora brassicae Wor.
PCR(Polymerase Chain Reaction)
detection
