摘要
目的 :探讨端粒酶活性水平检测对癌性胸腔积液的诊断价值。方法 :采用Telomerase PCR ELISA法检测了 30例癌性胸腔积液及 2 1例良性胸腔积液的端粒酶活性水平 ,并与细胞学检查比较。结果 :癌性胸腔积液端粒酶活性阳性率 76 .6 % ,结核性胸腔积液 2 0 % ( 3/15 ) ,其他病因胸腔积液 0 % ( 0 /6 ) ,癌性胸腔积液端粒酶活性阳性率高于细胞学检测率 36 .6 % (P <0 .0 0 5 ) ,两者平行试验阳性率 83.3%。结论 :端粒酶活性检测可能成为诊断癌性胸腔积液的重要辅助手段。
Objective:To investigate the diagnostic significance of the detection of telomerase activity in pleural effusion cells from patients with malignant neoplasm. Methods:The technique telomerase polymerase chain reaction(PCR)-enzyme-linked immunosorbent assay (ELISA) was employed to detect telomerase activity in 30 samples of pleural effusion cells of patients with malignant neoplasm, 20 samples of tuberculotic patients and 6 samples of patients with other diseases. Results:It was found that the positive rate of telomerase activity in samples from patients with malignant diseases were higher (76.6%) than that from tuberculotic patients(20%) or other patients(0%). The positive rate of telomerase activity in samples from patients with malignant diseases was much higher than that of cytologic measure. The combining positive rate of the two examinations was 83.3%. Conclusions:Detection of telomerase activity in pleural effusion cells would be helpful to the cytologic measure in diagnosis of patients with malignant neoplasm.
出处
《江苏大学学报（医学版）》
CAS
2002年第2期146-147,共2页
Journal of Jiangsu University：Medicine Edition
关键词
肿瘤
胸腔积液
端粒酶
Lung neoplasm
Pleural effusion
Telomerase
