摘要
目的 比较成年大鼠 4种脊髓全横断方法对后肢运动功能及脊髓组织学的影响 .方法 32只成年 SD大鼠分为A,B,C和 D 4组 ,每组 8只 ,分别以 4种不同方法全横切 T9脊髓 .A组以尖刀片自左向右横行一次性切断脊髓 ;B组将尖刀片自脊髓背部中线处垂直插入并分别向两侧缓慢细心切断脊髓 ;C组将一丝线穿过脊髓腹侧硬膜外腔 ,以长刃显微剪一次性完全横断脊髓 ,并将丝线由断端间隙中拉出 ;D组以尖刀片自脊髓背侧至腹侧分层快速划断脊髓 ,再抬起脊髓两断端以验证横断的完全性 .术后 1h肉眼观察脊髓形态变化 ,8wk时评估截瘫后肢自发性运动功能恢复后 ,处死动物 ,取脊髓损伤节段 ,行连续矢状冰冻切片 ,小鼠抗神经丝抗体免疫组化染色 ,光镜下观察有无神经纤维的残留或再生 ,以及空洞及瘢痕的形成 .结果 A,B两组术后脊髓肿胀、外翻 ,8wk时分别有 5 0 % ,38%的动物出现程度不等的功能恢复 ,镜下可见成束残留纤维和大量空洞 .C,D两组术后脊髓外观良好 ,8wk时无功能恢复和残留纤维 ,但有少量神经丝免疫反应再生纤维 .与 D组相比 ,C组断端间隙较大且空洞较多 .结论 经作者创新的 D组方法 ,横断完全、损伤较小 ,为简便易行。
AIM To compare the effects of four spinal cord transecting methods on spontaneous motor recovery in paraplegic hindlimbs and histological changes within the spinal cord in adult rats. METHODS Thirty two adult male SD rats were divided into Groups A, B, C and D ( n =8 for each group, which received one of the four methods of spinal cord transection at T9). In Group A, the spinal cord was transected with a sharp blade along the inner wall of the vertebral channel. The blade in Group B, however, was vertically inserted through the cord at midline and guided to slowly and carefully cut the bilateral halves of the cord separately. In Group C, the cord was cut with a pair of long edge microscissors, and the completeness of the transection was verified by guiding a surgical suture through the ventral extradural space before and pulling the suture out through the gap between the 2 stumps of the transected cord. The cord of Group D animals was dissected with a sharp blade in quick gashes, and complete transection was checked by uplifting one of the stumps. The appearance of the exposed spinal cord was observed with naked eyes 1 h after the surgery, and the locomotor recovery of the hindlimbs was evaluated with the BBB rating scale at 8 wk after surgery before sacrificing all animals. The damaged spinal cord segments were sectioned sagittally, prepared for anti neurofilement immunostaining, and observed under a light microscope for any possible remaining and regenerating nerve fibers as well as the formation of cavitation and scars. RESULTS Spinal cord edema and ectropion could be encountered 1 h after the surgery, and 50% and 38% animals showed different degrees of locomotor recovery and corresponding remaining nerve fibers with many scars and cavities 8 wk following the surgery in Groups A and B. As for Groups C and D animals, a better appearance of the cord without much edema and ectropion was observed at 1 h and no locomotor recovery could be revealed at 8 wk. A few neurofilament positive regenerating, but not remaining, fibers were observed within the lesion site. Group C animals had a wider gap between the two stumps of the transected spinal cord and more cavities in the injured spinal segments than those animals in Group D. CONCLUSION The authors' innovated transecting method in Group D is easy and reliable because of its properties of the completeness of transection and relatively milder injury to the spinal cord.
出处
《第四军医大学学报》
北大核心
2002年第11期965-968,共4页
Journal of the Fourth Military Medical University
基金
国家 973脑功能和脑重大疾病的基础研究基金资助课题(G19990 5 40 0 9)
关键词
脊髓切断术
神经纤维
轴突再生
大鼠
cordotomy
nerve fibers
cavities
axonal regeneration
rat
