摘要
目的:研究珍珠梅乙酸乙酯提取物对CCl_4所致大鼠急性肝损伤的保护作用。方法:给动物ig给药,用CCl_4造大鼠急性肝损伤模型,用比色分析法测定大鼠血清天冬氨酸转氨酶(aspartate transaminase,ALT)、丙氨酸转氨酶(alanine transaminase,AST)的活性;在大鼠血清、肝匀浆、肝线粒体测定超氧化歧化酶(superoxide dismutase, SOD)、谷胱甘肽过氧化物酶(glutathione perioxidase,GSH-PX)的活性,丙二醛(malondialdehyde,MDA)的含量;在肝线粒体测定琥珀酸脱氢酶(Succinate dehydrogenase,SDH)的活性。结果:珍珠梅能显著降低因CCl_4所致急性肝损伤大鼠血清ALT(300±50&1617±267 vs 4751±451,P<0.01)、AST(533±200&1767±217 vs 3800±383,P<0.01)的升高;明显回升CCl_4所致肝损伤大鼠肝线粒体SDH(104±18&47±10 vs 7±2,P<0.05)活性的降低;对急性肝损伤大鼠血清、肝匀浆、肝线粒体SOD(40±2&25±3 vs 14±6,P<0.05;50±8&31±9 vs 20±6,P<0.05;60±8&34±6 vs 22±9,P<0.05)、GSH-PX(73±21&59±14 vs 45±16,P<0.05;126±39&135±49 vs 89±21,P<0.05;206±39&193±50 vs 117±60,P<0.05)的活性有明显的升高作用及降低MDA(1.3±0.6&1.8±0.7 vs 2.7±0.8,P<0.05;1.3±0.4&4.0±1.7 vs 6.9±1.1,P<0.05;0.8±0.2&2.4±2.2 vs 5.1±1.7,P<0.01)的含量。结论:珍珠梅乙酸乙酯提取物对CCl_4所致大鼠急性肝损伤有保护作用。
AIM: To study the protective effects of Sorbaria sorbifolia extract on carbon tetrachloride induced acute hepatic injury in rats. METHODS: Sorbaria sorbifolia was administrated ig to the rats with induced acute hepatic injury by carbon tetrachloride. Colorimetric method was used to measure the activities of aspartate transaminase (AST), alanine transaminase (ALT), superoxide dismutase (SOD), succinate dehydrogenase (SDH) and glutathione perioxidase (GSH-PX), and the content of malondialdehyde (MDA). RESULTS: Sorbaria sorbifolia extract significantly reduced acute hepatic injury induced by carbon tetrachloride, with increasing serum ALT (300±50&1617±267 vs 4751±451, P<0.01) and AST(533±200&1767±217 vs 3800±383, P<0.01), and increased the activity of decreasing SDH(104±18&47±10 vs 7±2, P<0.05) in liver migocsondria. The activities of SOD (40±2&25±3 vs 14±6,P<0.05; 50±8&31±9 vs 20±6, P<0.05; 60±8&34±6 vs 22±9, P<0.05) and GSH-PX(73±21&59±14 vs 45±16, P<0.05; 126±39&135±49 vs 89±21, P<0.05; 206±39&193±50 vs 117±60, P<0.05)in serum, liver homogenate and mitochondria in the treatment group were higher than those in model group. The contents of MDA (1.3±0.6&1.8±0.7 vs 2.7±0.8, P<0.05; 1.3±0.4&4.0±1.7 vs 6.9±1.1, P<0.05; 0.8±0.2&2.4±2.2 vs 5.1±1.7, P<0.01) in the treatment group were lower than those in model group. CONCLUTION: Sorbaria sorbifolia extract has protective effects on rat acute hepatic injury induced by carbon tetrachloride.
出处
《世界华人消化杂志》
CAS
2002年第7期783-786,共4页
World Chinese Journal of Digestology
