摘要
目的 确定肾综合征出血热病毒 (HFRSV)在螨体内的定位。方法 用原位RT PCR技术检测HFRSV在螨体内的分布。结果 HFRSV多见于革螨及恙螨的卵巢卵细胞、中肠及中肠支囊上皮细胞的细胞核周围 ,呈弥散分布 ,前体组织细胞少见 ,其他组织细胞未见。革螨子 4代、子 3代和恙螨若虫阳性颗粒多于革螨子 2代、子 1代和恙螨幼虫。
Objective To study the distribution of hemorrhagic fever with renal syndrome virus (HFRSV) in mites Methods In situ reverse transcription polymerase chain reaction (IS RT PCR) was used for detecting the distribution of HFRSV in mites Results HFRSV RNA was mainly located in ovary and mid gut tissues of gamasid mites and chigger mites.The positive signal intensity in the third and fourth generations of gamasid mite was stronger than that in the first and second generations, and that in nymph of chigger mite more than larva Conclusion Both chigger mite and gamasid mite could play an important role in the transmission of HFRSV.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2002年第4期232-234,F003,共4页
Chinese Journal of Preventive Medicine
基金
国家自然科学基金资助 ( 39970 6 35)
江苏省自然科学基金资助 (BK99155)
