摘要
本文应用抗HFRSV McAb的APAAP(碱性磷酸酶—抗碱性磷酸酶)免疫组化技术,首次用光镜在HFRSV感染8—14天的Hep-2和wish细胞中查出病毒包涵体,并且根据其位置和形态特征可分为:(1)中央型包涵体;(2)外周型包涵体;(3)脱落包涵体。上述所见包涵体均在电镜下得到证实。此结果对于了解HFRSV感染细胞后的致病变作用以及研究HFRSV感染细胞包涵体的特征、来源、性质和形成过程具有十分重要的意义。
Investigation of the HFRSV inclusion bodies was carried out under ordinary optical microscope with APAAP(alkaline phosphatase anti-alkaline phosphatase)immunohistochemical technique in the Hep-2 and Wish cell lines infected by HFRSV (Hemorrhagic fever with Renal syndrome virus). It was found that there are three types of virus inclusion bodies, central, pheripheral and shedding types. All these findings were verified by electron microscopy, These results have great significance for further understanding the pathological changes of HFRSV infected cells~ and are very useful for studying on the morphological characterization, origin and formation of HFRSV inclu sion body.
出处
《中国病毒学》
CSCD
1991年第3期197-200,共4页
Virologica Sinica
基金
国家自然科学基金
