摘要
目的 :研究虎纹镇痛肽 1(HWAP 1)的致突变性。方法 :采用鼠伤寒沙门氏菌回复突变试验、中国仓鼠肺成纤维细胞染色体畸变试验、小鼠骨髓嗜多染红细胞微核试验研究了虎纹镇痛肽 1的致突变作用。结果 :Ames试验结果 :HWAP 1的 4个剂量组 (1μg/皿～ 10 0 0 μg/皿 ) ,在活化及非活化条件下诱发TA97、TA98、TA10 0和TA10 2菌株的回变菌落数未见明显增加 ;染色体畸变试验结果 :HWAP 1的 4个剂量组 (2 6 3μg/皿～ 2 10 0 μg/ml) ,在活化及非活化条件下CHL细胞染色体畸变率小于 5 % ;微核试验结果 :HWAP 1在 10 0 μg/皿～ 4 6 0 μg/kg剂量范围内 ,小鼠骨髓嗜多染红细胞微核率未见明显增加。结论 :在本试验条件下 ,HWAP 1无致突变作用。
Purpose: To study the mutagenicity of HUWEN analgesic peptide 1(HWAP 1). Methods: Ames test, chromosome aberration test of Chinese hamaster lung cell and micronucleus test of PCE in mouse bone marrow were used. Results: The Ames test results showed that the reversion mutation frequencies of HWAP 1 with four doses 1～1 000 (μg/plate) did not increase in strain TA97,TA98,TA100 and TA102 with or without S\-\{9mix\}. In the doses of 263～2 100 (μg/ml), the frequencies of chromosome aberration of CHL cell were less than 5 % with or without S\-\{9mix\}. In the doses of 100～460 (μg/kg), the frequencies of micronucleus in bone marrow PCE cells of mice did not increase. Conclusion: HWAP 1 had no mutagenicity at the doses used.
出处
《癌变．畸变．突变》
CAS
CSCD
2002年第3期174-177,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金资助项目 (3 93 70 15 2 )
