摘要
目的 :探讨 p5 3和 k- ras基因突变在大肠癌中的临床意义。方法 :采用 PCR- SSCP方法检测 64例大肠癌患者癌组织及癌旁正常粘膜组织的 p5 3和 k- ras基因。结果 :64例大肠癌患者中发生 p5 3基因突变 2 8例 ( 4 3.8% ) ,发生 k- ras基因突变 2 6例 ( 4 0 .6% ) ,出现 p5 3和 kras基因协同突变 1 1例 ( 1 7.2 % )。p5 3和 k- ras基因突变与病人性别、肿块部位、大小、浸润深度及 Dukes分期无关 ;k- ras基因突变组年龄略高于非突变组 ;p5 3或 k- ras基因单一突变组易出现淋巴结转移 ,但无统计学意义 ;p5 3和 k- ras基因协同突变组淋巴结转移率 ( 1 0 /1 1、90 .9% )明显高于两者均无突变组( 7/2 1、33.3% ,P<0 .0 5 )。结论 :p5 3和 k- ras基因协同突变可能直接或间接促进了大肠癌的进一步发展。
Objective: To study on the clinical significance of p53 and k ras mutations in carcinoma of colon and rectum. Methods: Detecting p53 and k ras mutations in the tissues of both carcinoma and the normal mucosa nearby by PCR SSCP in 64 patients with colon or rectum carcinoma. Results: Mutations of p53 and k ras were found in 28 (43 8%) and 26 among 64 cases (40 6%),respectively, and simultaneous mutations of p53 plus k ras were observed in 11 from of 64 cases. Based on the analysis of correlation with the clinical aspects, it was found that p53 and k ras mutations were not associated with sex of patients, the situs , size, depth of infiltration or Dukes staging of the carcinoma. Age in the k ras mutation group was a little older than that of the control, the ratio of lymph node metastasis in the group with p53 or k ras mutation was a little higher than that in the control, the ratio of lymph node metastasis in the p53 plus and k ras (10/11,90 9%) simultaneous mutation was group remarkably higher than that of the group without combining mutations (7/21,33 3%, P <0 05).Conclusion: Simultaneous mutations of p53 and k ras may promote the progression of colon and rectum carcinoma directly or indirectly.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2002年第4期392-394,共3页
Journal of Jilin University：Medicine Edition
