摘要
为分析羊口疮病毒ORFV121和vIL-10基因在凤翔(FX0910)强毒株(vORFV)和弱毒株(lvORFV)之间的差异,应用PCR方法扩增羊口疮病毒ORFV121和vIL-10基因的全长序列并测序,应用生物信息学软件分析基因的核酸、氨基酸相似性,蛋白的亲水性、二级结构以及抗原性。结果显示,lvORFV的ORFV121基因118位的谷氨酸缺失,说明该处发生的碱基突变为有意突变,该突变导致弱毒株相应蛋白的亲水性、二级结构发生变化,蛋白的抗原性在很多区域发生本质改变;vIL-10在蛋白质水平出现了5个氨基酸位点突变(14:V→W,49:A→P,57:T→M,72:R→C,102:I→V)和79位插入1个色氨酸。这些位点差异导致蛋白的亲水性、二级结构和抗原性发生了变化。ORFV121基因和vIL-10基因在vORFV和lvORFV之间存在差异,这些基因差异导致蛋白的亲水性、二级结构和抗原性发生改变。
In order to analyse the diffenences in ORFV121 gene and vIL-10gene between the virulent and low virulent strains of ORFV-FX0910.The full lengths of the two genes were abtained by the method of PCR amplification and then for sequencing.The similarities of nucleic acids,amino acids,hydrophilicities,secondary structures,and antigenicities of proteins were analyzed using bioinformatics software.The results showes that,there was a glutamic deletion at site 118 of ORFV121in the lvORFV,which suggested the corresponding base mutation was significant.As a result,hydrophilicity and secondary structure were changed,and antigenicity was changed essentially at many domains of the ORFV121 protein.For vIL-10protein,there were 5amino acid mutations（14：V→W,49：A→P,57：T→M,72：R→C,102：I→V）and one tryptophan insertion at site 79,which resulting in the variations of hydrophilicity,secondary structure,and antigenicity.In conclusion,there were differnences in ORFV121 gene and vIL-10gene between vORFV and lvORFV,and the hydrophilicities,secondary structures,and antigenicities of proteins were changed due to these mutations.
出处
《动物医学进展》
CSCD
北大核心
2014年第7期52-58,共7页
Progress In Veterinary Medicine
基金
陕西省科技统筹新工程计划项目(2013KTZB02-02-02
2013KTZB02-02-03)
