摘要
在实验室条件下,设置0.1、0.5、1.0 mg/L3个Cu离子浓度组和1个空白对照组,研究了Cu离子对体质量为(803.1±35.5)g的杂交鲟(欧洲鳇Huso huso Linnaeus♀×小体鲟Acipenser ruthenus Linnaeus♂)的行为指标、血液指标和血清中抗氧化酶活性的影响。试验采样分24、48、72 h 3个时间段。结果表明:Cu离子对杂交鲟的行为指标和血液参数有相同的影响趋势,即随着Cu离子浓度的增大,杂交鲟的呼吸频率和摆尾频率逐渐增大,血清中的红细胞数和血红蛋白含量逐渐增多,但随着暴露于Cu离子中时间的延长,4种指标逐渐降低;Cu离子对杂交鲟白细胞数以及CAT和SOD酶活性的影响表现出一定的剂量效应关系,即随着Cu离子浓度的增大,白细胞数以及CAT和SOD活性呈先升高后降低,而在相同Cu离子浓度下,随着暴露于Cu离子中时间的延长,白细胞数和抗氧化酶活性均明显降低。研究表明,低浓度Cu离子对杂交鲟抗氧化酶活性和免疫细胞有明显的诱导作用,而高浓度Cu离子则抑制其免疫和抗氧化功能。
The influence of Cu2＋ concentration（ 0,0.1,0.5 and 1.0 mg/L） on behavior,blood parameters and antioxidant enzyme activity was studied in hybrid strugeon（ European huso sturgeon Huso huso Linnaeus ♀ × sterlet Acipenser ruthenus Linnaeus ♂） with body weight of（ 803.1 ± 35.5） g for 24 h,48 h and 72 h in a laboratory.The results showed that respiratory frequency,tail beating frequencies,and levels of red blood cell（ RBC） and hemoglobin were increased with increase in concentration of Cu2＋,and then decreased with Cu2＋ exposure elapse.The number of white blood cell（ WBC）,and catalase（ CAT） and superoxide dismutase（ SOD） activities were found to be dose-dependent,first increase,and then decrease with increase in concentrations of Cu2＋.In the same concentration of Cu2＋,however,the number of WBC and activities of antioxidant enzymes were decreased with Cu2＋ exposure elapse.The findings indicate that low concentration of Cu2＋ leads to induction of activities of antioxidant enzymes and activation of immune cells while high level of Cu2＋ results in inhibition of immune cells and activities of antioxidant enzymes.
出处
《大连海洋大学学报》
CAS
CSCD
北大核心
2014年第3期241-245,共5页
Journal of Dalian Ocean University
基金
河北省科技支撑计划项目(10220505)
河北农业大学青年基金资助项目(QJ201205)
广东普通高校重点实验室开放基金资助项目(J201304)
关键词
杂交鲟
铜离子
行为指标
血液指标
抗氧化酶
hybrid strugeon
copper ion
behavior index
blood index
antioxidant enzyme
