摘要
目的：研究制痂酊工艺及质量标准。方法：按改进工艺制备制痂酊。采用高效液相色谱法同时测定制痂酊中盐酸小檗碱、黄芩苷含量，采用C18（Inertsil ODS-SP5um 4.6mm×150mm）为固定相；乙腈∶0.1%磷酸（45∶55含十二烷基磺酸钠）为流动相。检测波长：265nm；流速：1.0ml·min-1，柱温：室温。结果：黄芩浓度在0.93765～7.500mg·ml-1，范围内线性关系良好，r=0.9996；盐酸小檗碱浓度0.456～3.720mg.ml范围内线性关系良好，r=0.9997。结论：制备方法操作简便，含量测定快速准确，可用于制痂酊的制备及质量控制。
Objective：To study the system the scab tincture technology and quality standards. Methods：scab tincture to improve technology preparation system. HPLC was used to simultaneous determination of the content of the system scab tincture of berberine, baicalin in C18 （Inertsil ODS-SP5um 4.6mm ×150mm） as the stationary phase; 0.1% phosphoric acid （45∶55 containing dodecane sulfonate） as the mobile phase. Detection wavelength：265nm;flow rate：1.0ml·min-1, column temperature：room temperature. Results：The concentration of skullcap good 0.93765-7.500mg·ml-1, linear relationship,r=0.9996; concentration of berberine hydrochloride in the range of 0.456-3.720mg·ml linear relationship （r=0.9997）. Conclusions：Of the preparation method is simple, fast and accurate content determination can be used for the preparation and quality control of the system scab tincture.
出处
《北方药学》
2014年第8期15-16,共2页
Journal of North Pharmacy
关键词
制痂酊
工艺改进
质量标准
盐酸小檗碱黄芩苷
高效液相色谱法
System of scab tincture
Process improvement
Quality standards Berberine hydrochloride
Baicalin
High performance liquid chromatography
