摘要
目的检测和分析细胞外信号调节激酶5(ERK5)在人胃腺癌组织和癌旁正常胃黏膜组织中蛋白和mRNA表达情况,探讨ERK5表达的临床意义。方法选取126例胃腺癌组织(胃腺癌组)和90例癌旁正常组织(正常组),采用免疫组织化学法检测两组ERK5蛋白表达,RT-PCR法检测ERK5 mRNA相对表达量,并分析ERK5蛋白、mRNA表达与胃腺癌临床病理参数的相关性。结果胃腺癌组及正常组ERK5蛋白表达阳性率及mRNA的相对表达量分别为92.8%、72.2%和(0.82±0.14)、(0.61±0.03),两组ERK5蛋白和mRNA表达差异有统计学意义(P<0.05)。不同的病理分级分期的胃腺癌ERK5蛋白和mRNA表达差异有统计学意义(P<0.05),而不同的性别、年龄的胃腺癌ERK5蛋白、mRNA表达差异无统计学意义(P>0.05)。Sperman等级相关分析法分析表明,ERK5蛋白和mRNA表达呈正相关(r=0.835,P<0.01)。结论胃腺癌组织中ERK5蛋白较癌旁正常组织高表达;分期越高分化程度越低及发生淋巴结转移的胃腺癌组织中ERK5的表达越高;提示ERK5和胃腺癌的恶性程度和转移密切相关。
[Objectives] To detect and analyze extracellular signal regulated kinase 5 （ERKS） expression of protein in human gastric adenocarcinoma tissue and adjacent normal gastric mucosa tissues and mRNA, to investigate the clinical significance of ERK5 expression. [Methods] Selected 126 cases of gastric adenocarci- noma （gastric adenocarcinoma group） and 90 cases of adjacent normal tissue （normal group）, immunohistochemical expression of ERK5 protein was detected in two groups, the relative expression amount detection ERK5 mRNA RT-PCR method, correlation and analysis of ERK5 protein, mRNA expression and clinicopatho- logical parameters of gastric adenocarcinoma. [Results] The relative expression in gastric adenocarcinoma group and the normal group, the positive rates of ERK5 protein and mRNA were respectively 92.8%, 72.2% and （0.82 ＋ 0.14, 0.61 _＋ 0.03）, two group of ERK5 protein and mRNA expression were significantly different （P 〈0.05）. Pathological grading of different stages of gastric adenocarcinoma ERK5 protein and mRNA expression were significantly different （P 〈0.05）, and the different gender, age of gastric adenocarcinoma ERK5 protein, mRNA expression had no significant difference （P〉0.05）. Analysis of Sperman rank correlation analysis, ERK5 protein and mRNA expression were positively correlated （r=0.835, P〈0.01）. [Conclusion] High expression in gastric adenocarcinoma tissue ERK5 than the adjacent normal tissues; the higher expression of staging and less differentiated and ERK5 lymph node metastasis in gastric adenocarcinoma is high; ERK5 and gastric adenocarcinoma malignant degree and metastasis.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2014年第20期56-60,共5页
China Journal of Modern Medicine
基金
国家自然科学基金(No:81160012)
关键词
胃腺癌
细胞外信号调节激酶5
蛋白和mRNA表达
gastric adenocarcinoma
extracellular signal-regulated kinase 5
expression of protein and mRNA
