摘要
参照《中国农作物种子检验规程》对太子参种子扦样、净度、千粒重、含水量、生活力、发芽情况等进行研究,筛选适合太子参种子品质的检验方法;并对不同产地太子参种子进行检验。研究结果表明,太子参种子最少试验量应为6.5 g,过10目筛后进行净度分析,千粒重测定以五百粒法,水分测定以高恒温(130±2)℃烘干5 h,生活力测定以0.2%TTC溶液40℃染色1 h,种子解除休眠后置于砂上10℃进行发芽试验;采用K聚类对各项数据进行分析,不同产地种子质量被分为3个等级,其中Ⅰ级种子的发芽率不低于86%、千粒重不低于2.59 g、净度不低于87%、含水量不高于13.1%,Ⅱ级种子的发芽率不低于70%、千粒重不低于2.40 g、净度不低于77%、含水量不高于14.3%,Ⅲ种子的发芽率不低于41%、千粒重不低于2.29 g、净度不低于76%、含水量不高于15.8%。该研究建立了太子参种子品质的检验方法和质量分级标准。
Referring to the rules for agricultural seed testing（ GB /T 3543-1995） issued by China,the test of sampling,seed purity,weight per 1 000 seeds,seed moisture,seed viability and germination rate had been studied for screening seed quality test methods of Pesudostellaria heterophylla. The seed quality from different collection areas was measured. The results showed that at least6. 5 g seeds should be sampled and passed through 10-mesh sieve for purity analysis. The weight of 1 000 seeds was determined by using the 500-seed method. The phenotypic observation and size measurement were used for authenticity testing. The seed moisture was determined under the higher temperature（ 130 ± 2） ℃ for 5 hours. The seeds were dipped into 0. 2% TTC sustaining 1 hour at 40 ℃,then the viability could be determined. The break dormancy seeds were cultured on sand at 10 ℃. K cluster analysis was applied for the data analysis,the seed quality from different collection areas grading of P. Heterophylla was described as three grades. The seed quality of each grade should reach following requirements： for first grade seeds,germination rate≥86%,1 000-grain weight≥2. 59 g,purity≥87%,moisture≤13. 1%; for second grade seeds,germination rate≥70%,1 000-grain weight≥2. 40 g,purity≥77%,moisture≤14. 3%; for third grade seeds,germination rate≥41%,1 000-grain weight≥2. 29 g,purity≥76%,moisture≤15. 8%.The seed testing methods for quality items of P. heterophylla had been initially established,as well as the primary P. heterophylla seed quality classification standard.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2014年第16期3042-3047,共6页
China Journal of Chinese Materia Medica
基金
国家"重大新药创制"科技重大专项(2012ZX09304006)
中央本级重大增减支项目(20603020223
20603020120)
施秉中药材产业科技合作专项计划项目[施科合专项(2012)04号]
贵州省普通高等学校特色重点实验室建设项目(黔教合KY字[2013]108号)
关键词
太子参
种子
品质检验方法
质量标准
Pesudostellaria heterophylla
seed
quality test methods
quality classification standard
