摘要
目的：制定白花蛇舌草药材质量标准,为药用植物资源的开发利用提供科学依据。方法：采集或收集22批白花蛇舌草药材,对这些样品的性状、检查、鉴别、指标成分含量进行了研究;采用薄层色谱（TLC）法进行定性鉴别;采用高效液相法测定去乙酰车叶草酸甲酯的含量。结果：对白花蛇舌草药材的性状、显微特征进行了描述;根据22个不同产地白花蛇舌草测定结果,确定白花蛇舌草水分不得超过6%,总灰分不得超过13%,酸不溶性灰分不得超过5%,浸出物以水为溶剂,热浸法测定,不得低于10%;TLC斑点清晰,分离度好;去乙酰车叶草酸甲酯的进样量在0.019～2.44μg（r=1.000 0）与峰面积积分值呈良好线性关系,平均回收率为98.28%,RSD 2.73%,去乙酰车叶草酸甲酯含量不得少于0.1 mg·g^-1。结论：该标准可用于白花蛇舌草药材的质量控制。
Objective： To establishing the quality control standard and provide scientific basis for the utilization and development of Hedyotidis diffusa. Method： Twentytwo batches of H. diffusa were collected. The morphology,inspection,identification and the content of target components in these crude drugs were studied. TLC method was used to identify this crude drug. The contents of deacetyl asperulosidic acid methyl ester in these samples were determined by HPLC. Result： Macroscopic and microscopic characteristics of H. diffusa were described. According to the measurement results,moisture in the crude drug was equal or below 6%,the content of total ash was equal or below 13 %,the content of acid-insolubal ash was equal or below 5%. The extractum by hot water was above 10%. The spot of TLC were clear and well-aparated. The linear range of deacetyl asperulosidic acid methyl ester were 0. 019-2. 44 μg（r = 1. 000 0）; the average recoveries were 98. 28% and RSD of them were2. 73（n = 6）; the content of deacetyl asperulosidic acid methyl ester were above 0. 1 mg·g^-1. Conclusion： The quality is suitable for H. diffusa.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第17期98-101,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
<中国药典>一部2015年版项目
北京自然科学基金项目(7112097)
中国中医科学院自主选题项目(ZZ2014016)
关键词
白花蛇舌草
质量标准
去乙酰车叶草酸甲酯
Hedyotidis diffusa
quality standard
deacetyl asperulosidic acid methyl ester
