摘要
为了解新疆塔城某规模化养殖场分离的大肠埃希菌对临床常用抗菌药物的耐药情况,从该规模化养殖场中采集的水样、饲料样、牛粪样及羊粪样中分离大肠埃希菌。采用微量肉汤法检测其对抗菌药物的耐药情况。结果表明,采集牛源饮用水样35份,分离率100.0%(35/35),分离的大肠埃希菌仅对阿莫西林/克拉维酸(31.4%)和氨苄西林(20.0%)2种抗菌药物耐药;牛源饲料样15份,分离率86.7%(13/15),分离的大肠埃希菌对氨苄西林(30.8%)、阿莫西林/克拉维酸(23.1%)、安普霉素(15.4%)、诺氟沙星(7.7%)、恩诺沙星(7.7%)和庆大霉素(7.7%)6种抗菌药物耐药;牛粪样20份,分离率100.0%(20/20),分离的大肠埃希菌对氨苄西林(60.0%)、阿莫西林/克拉维酸(50.0%)、恩诺沙星(40.0%)、庆大霉素(40.0%)、头孢噻呋(35.0%)、阿米卡星(25.0%)、诺氟沙星(10.0%)和环丙沙星(10.0%)8种抗菌药物耐药;羊粪样55份,分离率100.0%(55/55),分离的大肠埃希菌对阿莫西林/克拉维酸(25.5%)、氨苄西林(12.7%)、庆大霉素(5.5%)、头孢噻呋(3.6%)、诺氟沙星(1.8%)、恩诺沙星(1.8%)和阿米卡星(1.8%)7种抗菌药物耐药。新疆塔城牛源大肠埃希菌对常用抗菌药物多药耐药情况较严重,临床用药需谨慎,且可能存在粪源菌污染水源和饲料的风险。
In order to investigate commonly used antimicrobial drug resistance to Escherichia coli isolates from a farm in Tacheng, Xinjiang,the minimal inhibitory concentrations （MIC） of the antimicrobial drugs to these isolates from drinking water,feed,bovine feces and ovine feces were determined by the broth micro-dilution method. The results showed that： 35 E. coli isolates were confirmed from 35 drinking water samples （100. 0%）, the resistance rates to amoxicillin/clavulanic acid and ampicillin were 31. 4% and 20%, respectively. 13 E. coli isolates were confirmed from 15 feed samples （86.7%）, the resistance rates to ampicillin, amoxicillin/clavulanic acid, apramycin, norfloxacin, enrofloxacin and gentamicin were 30.8%, 23.1%, 15.4%, 7.7%, 7.7% and 7.7%, respectively. 20 E. coli isolates were confirmed from 20 bovine fecals samples （100.0%）, the resistance rates to ampicillin, amoxicillin/clavulanic acid, enro- floxacin, gentamicin, ceftiofur, amikacin, norfloxacin and ciprofloxacin were 60.0%, 50. 0%, 40.0%, 40.0%, 35.0%, 25.0%, 10.0% and 10.0%, respectively. 55 E. coli isolates were confirmed from 55 ovine fecals samples（100.0%）, the resistance rates to amoxicillin/clavulanic acid, ampicillin, gentamicin, ceftiofur, norfloxacin, enrofloxacin and amikacin were 25.5%, 12.7%, 5.5%, 3.60/6, 1.8%, 1.8% and 1.8%, respectively. The multi-drug resistance of E. coli is serious in Tacheng Xinjiang. The clinical medi- cation needs to be careful, and there may be a risk of fecal bacterial pollution to water and feed.
出处
《动物医学进展》
CSCD
北大核心
2014年第9期118-123,共6页
Progress In Veterinary Medicine
基金
国家自然科学基金项目(31260614)
新疆维吾尔自治区普通高校重点学科(基础兽医学)科研启动基金项目
关键词
大肠埃希菌
抗菌药物
耐药
微量肉汤法
Escherichia coli
antimicrobial
drug resistance
broth method
