摘要
目的:观察针刺百会、内关穴对大脑中动脉阻塞(MCAO)大鼠未折叠蛋白反应(UPR)通路eIF2α因子基因表达的影响,探讨针刺抑制细胞凋亡、保护脑神经元的内在机制。方法:将SD大鼠60只随机分为捆绑组、假手术组、模型组、针刺组、依达拉奉组,每组12只。线栓法制备MCAO大鼠脑缺血再灌注模型,RT-PCR法检测缺血侧顶叶大脑皮层eIF2α基因表达变化。结果:与捆绑组和假手术组比较,模型组、针刺组及依达拉奉组eIF2α表达明显增高(P<0.01或P<0.05);与模型组比较,针刺组及依达拉奉组eIF2α基因表达显著降低(P<0.01);与依达拉奉组比较,针刺组eIF2α表达变化差异无统计学意义(P>0.05)。结论:针刺可以下调脑缺血大鼠未折叠蛋白反应通路eIF2α因子基因的表达,这可能是针刺发挥脑保护作用的内在机制之一。
Objective：To explore the protection mechanism of the acupuncture on transient MCAO rat mode through investigating the effects of the acupuncture on eIF2α genes expression in Unfold Protein Response（UPR） signal pathway. Methods：The 60 rats were randomly divided into bundle group, sham-operation group,model group,acupuncture group, and annaoping-chong pill group, 12 in each. Silk was used for ligation of the middle cerebral artery of rats, to establish MCAO rats mode. Methods of reverse transcription-polymerase chain reaction （RT-PCR） were used to measure the expression of eIF2α mRNA. Results： Compared with the bundle group and sham-operation group, the expression of eIF2α were up-regulated in peri-infarction after cerebral ischemia/reperfusion in model group,acupuncture group and annaoping-chong pill group （P〈0.01 or P〈0.05）.The expression level of eIF2α mRNA in intervention models were reduced accordingly （P〈0.01）. Compared with the model group,the expression of eIF2α were down-regulated in peri-infarction after cerebral ischemia/reperfusion in acupuncture group and annaoping -chong pill group （P〈0.01 or P〈0.05）; Compared with the annaoping-chong pill group, the changes of expression level of eIF2α mRNA in acupuncture group were not statistically significant （P〉0.05）. Conclusion：Acupuncture can down-regulated the expression of eIF2α genes in Unfold Protein Response（UPR） signal pathway of MCAO rat mode, and this may be one of the protection mechanism of acupuncture treatment.
出处
《中医药导报》
2014年第10期11-14,共4页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金资助项目(81202771)
教育部博士点新教师类项目资助(20114323120005)
关键词
脑缺血
EIF2Α
未折叠蛋白反应
针刺
Cerebral ischemia/reperfusion
eIF2α
Unfolded protein response（UPR）
Acupuncture
