期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

miR-21对绝经后骨质疏松骨髓间充质干细胞成骨能力影响的研究

Influence of miR-21 on osteogenic capability in human bone marrow mesenchymal stem cells in patients with postmenopausal osteoporosis
在线阅读 下载PDF
导出
摘要 目的 探讨miR-21对绝经后骨质疏松骨髓间充质干细胞（PMOP-hBMMSC）成骨能力影响.方法 分离培养正常人（H-hBMMSCs）和绝经后骨质疏松患者（PMOP-hBMMSCs）的骨髓间充质干细胞,并对两组细胞的成骨能力进行比较.转染上调PMOP-hBMMSCs中miR-21表达,观察分析其对成骨能力的影响.Real time RT-PCR和Western Blot比较miR-21在三组细胞间的表达差异,同时检测三组成骨标志性基因Runx2和Osterix的表达.结果 PMOP-hBMMSCs组中的miR-21表达水平、ALP的活性以及钙结节染色面积都高于PMOP组（P＜0.05）,与H-hBMMSCs正常对照组差异无统计学意义（P＞0.05）;RT-PCR和Western Blot结果显示成骨诱导7d后,转染后高表达Runx2和Osterix mRNA和蛋白水平均高于PMOP组（P＜0.05,P＜0.01）,与H-hBMMSCs正常对照组差异无统计学意义（P＞0.05）.结论 转染miR-21后的PMOP-hBMMSCs成骨能力增强. Objective To investigate the osteogenic capability of miR-21 in human bone marrow mesenchymal stem cells in patients with postmenopausal osteoporosis （PMOP-hBMMSCs）.Methods The human bone marrow mesenchymal stem cells （hBMMSCs） and PMOP-hBMMSCs were isolated and cultured.The osteogenic capabilities of these two kinds of cells were compared.After over-expression miR-21,the osteogenic potential was analysed in PMOP-hBMMSCs.The expression of Runx2 and Osterix,genes in H-hBMMSCS and PMOP-hBMMSCs were respectively detected by real time RT-PCR and western blot.Results The expression level of miR-21 was higher,ALP was more active,and the calcium nodules staining area was larger in the PMOP-hBMMSCs group than in the PMOP group（P 〈 0.05）.Up-regulation was found in the expressions of Runx2 and Osterix mRNA and protein of Over-expression of miR-21 PMOP-hBMMSCs compared to PMOP（P 〈 0.05,P 〈 0.01）.Conclusion After the transfection of miR-21,the osteogenic ability of PMOP-hBMMSCs can be enhanced.
作者 廖壮文 黄彦 范子文 吴波以
机构地区 广州医科大学附属第二医院骨外科
出处 《国际医药卫生导报》 2014年第18期2816-2819,共4页 International Medicine and Health Guidance News
关键词 MIR-21 绝经后骨质疏松骨髓间充质干细胞 转染 miR-21 PMOP-hBMMSCs Transfection
分类号 R580 [医药卫生—内分泌]
  • 相关文献

参考文献14

  • 1Gonzalez-Rey E, Gonzalez MA, Varela N, et al. Human adipose-derived mesenchymal stem cells reduce inflammatory and T cell responses and induce regulatory T cells in vitro in rheumatoid arthritis [J]. Ann Rheum Dis, 2010, 69 (1) : 241-248.
  • 2Guerit D, Maumus M, Apparailly F, et al.Therapeutic mesenchymal stem or stromal cells in rheumatic diseases: rationale, clinical data and perspectives[J]. Clinical Investigation, 2011, 1 (9) : 1269-1277.
  • 3Le LT, Swingler TE, Clark IM. Review: the role of microRNAs in osteoarthritis and chondrogenesis [J]. Arthritis Rheum, 2013, 65 (8) : 1963-1974.
  • 4Ragni E, Montemurro T, Montelatici E, et al. Differential microRNA signature of human mesenchymal stem cells from different sources reveals an "environmental-niche memory" for bone marrow stem cells [J]. Exp Cell Res, 2013, 319 (10) : 1562-1574.
  • 5Tian H, Guo M, Zhuang Y, et al. Enhanced proliferation of bone marrow mesenchymal stem cells by co-culture with TM4 mouse Sertoli cells: involvement of the EGF/ PI3K/AKT pathway [J]. Mol Cell Biochem, 2014, 393 (1-2) : 155-164.
  • 6Pan GZ, Yang Y, Zhang J, et al. Bone marrow mesenchymal stem cells ameliorate hepatic ischemia/ reperfusion injuries via inactivation of the MEK/ERK signaling pathway in rats [J].J Surg Res, 2012, 178 ( 2 ) : 935-948.
  • 7Gentile A, Toietta G, Pazzano V, et al. Human epicardium-derived cells fuse with high efficiency with skeletal myotubes and differentiate toward the skeletal muscle phenotype : a comparison study with stromal and endothelial cells [J].Mol Biol Cell, 2011, 22 ( 5 ) : 581-592.
  • 8Li Y, Fan L, Liu S, et al. The promotion of bone regeneration through positive regulation of angiogenic- osteogenic coupling using microRNA-26a [J]. Biomaterials, 2013, 34 (21) : 5048-5058.
  • 9Zhang F, Jing S, Ren T, et al. MicroRNA-10b promotes the migration of mouse bone marrow-derived mesenchymal stem ceils and downregulates the expression of E-cadherin [J].Mol Med Rep, 2013, 8 (4) : 1084-1088.
  • 10Mao J, Lv Z, Zhuang Y. MicroRNA-23a is involved in tumor necrosis factor-eL induced apoptosis in mesenchymal stem cells and myocardial infarction [J].Exp MolPathol, 2014, 97 (1) : 23-30.

二级参考文献18

  • 1Fisher CJ Jr, Slotman G J, Opal SM, et al.Initial evaluation of human recombinant interleukin-1 receptor antagonist in the treatment of sepsis syndrome: a randomized, open-label, placebo-controlled multicenter trial[J].Crit Care Med, 1994, 22(1): 12-21.
  • 2Taganov KD, Boldin MP, Baltimore D.MicroRNAs and immunity: tiny players in a big field[J].Immunity, 2007, 26(2): 133-137.
  • 3Taganov KD, Boldin MP, Chang KJ, et al.NF- kappaB-dependent induction of microRNA miR-146, an inhibitor targeted to signaling proteins of innate immune responses[J].Proc Natl Acad Sci U S A, 2006, 103(33): 12481 - 12486.
  • 4Jing Q, Huang S, Guth S, et al.Involvement of microRNA in AU-rich element-mediated mRNA instability[J].Cell, 2005, 120(5): 623-634.
  • 5Yu F, Yao I-I, Zhu P, et al.let-7 regulates serf renewal and tumorigenicity of breast cancer cells[J].Cell, 2007, 131(6): 1109-1123.
  • 6E1 Gazzar M, McCall CE.MicroRNAs distinguish translational from transcriptional silencing during endotoxin tolerance[J].J Biol Chem, 2010, 285(27): 20940-20951.
  • 7Huang B, Zhao J, Lei Z, et al.miR-142-3p restrictscAMP production in CD4+CD25- T cells and CD4+CD25+ TREG cells by targeting AC9 mRNA[J].EMBO Rep, 2009, 10(2): 180-185.
  • 8Defer N, Best-Belpomme M, Hanoune J.Tissue specificity and physiological relevance of various isoforms of adenylyl cyclase[J].Am J Physiol Renal Physiol, 2000, 279(3): F400-416.
  • 9Li LM, Hou DX, Guo YL, et al.Role of microRNA- 214-targeting phosphatase and tensin homolog in advanced glycation end product-induced apoptosis delay in monocytes[J].JImmunol, 2011, 186(4): 2552-2560.
  • 10Sun Y, Varambally S, Maher CA, et al.Targeting of microRNA-142-3p in dendritic cells regulates endotoxin- induced mortality[J].Blood, 2011, 117(23): 6172-6183.
国际医药卫生导报
2014年 第18期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部