摘要
目的神经系统疾病与神经细胞的凋亡密切相关。文中旨在探讨牛磺酸通过活化Caspase 9对海马神经元细胞凋亡的抑制作用,进而探讨牛磺酸对神经系统的保护作用及其机制。方法海马神经元细胞分为4组:对照组、损伤凋亡组、牛磺酸低剂量保护组、牛磺酸高剂量保护组。监测各组细胞生长状态,MTT监测各组细胞凋亡状态,免疫荧光及蛋白印迹法测定Caspase 9在各组中的表达水平。结果与对照组比较,损伤凋亡组海马神经元细胞生长不良,MTT实验示损失凋亡组细胞活力(A值为0.102±0.025)明显低于对照组(A值为0.643±0.013),低、高剂量干预组细胞活力(A值分别为0.504±0.072、0.452±0.029)明显提高(P<0.05);免疫荧光测定示损伤凋亡组Caspase 9活化明显增高(A值为61 386.8±10 083.6),对照组(A值为4 502.2±2 518.1)及牛磺酸低、高剂量保护组(A值分别为20 077.4±4 187.5和13 976.2±7 044.1)活化较低(P<0.05);蛋白印迹法示损伤凋亡组Caspase 9表达(A值为1.23)较对照组(A值为0.17)及低、高剂量保护组(A值分别为0.21和0.19),明显升高(P<0.05)。结论牛磺酸可抑制Caspase 9的活化,对神经细胞有较好的保护作用。
Objective Neurological diseases are closely associated with the apoptosis of neuronal cells .This article aims to study the inhibitory effect of taurine on the apoptosis of hippocampal neurons by activating Caspase 9 as well as its protective effect on the nervous system and its mechanisms . Methods Mouse hippocampal neuronal cells were randomly divided into four groups：control, injury and apoptosis, low-dose taurine protection, and high-dose taurine protection.The proliferation of the neuronalcells was observed, their apoptosis examined by MTT colorimetric assay, and the expression of Caspase 9 in different groups detected by immunofluorescence and Western blot. Results The injury and apoptosis group showed a poor proliferation of the hippocampal neuronal cells and decreased cell viability （A=0.102 ±0.025）, significantly lower than the control group （relative A=0.643 ± 0.013）, the low-dose taurine group （relative A=0.504 ±0.072）, and the high-dose taurine group （relative A=0.452 ±0.029） （ all P〈0 .05 ） .Immunofluorescence assay revealed significantly increased Caspase 9 activation in the injury and apoptosis group （A=61386.8 ±10083.6） compared with the control （A=4502.2 ±2518.1）, the low-dose taurine （A=20077.4 ±4187.5）, and the high-dose taurine group （A=13976.2 ±7044.1） （all P〈0.05）.Western blot showed a remarkably higher expression of Caspase 9 in in the injury and apoptosis group （A=1.23） than in the control （relative A=0.17）, the low-dose taurine （A=0.21）, and the high-dose taurine group （A=0.19） （all P〈0.05）. Conclusion Taurine can protect neuronal cells by inhibi-ting Caspase 9 activation.
出处
《医学研究生学报》
CAS
北大核心
2014年第8期793-796,共4页
Journal of Medical Postgraduates
基金
国家自然科学基金(81360183)
