摘要
目的 探讨电子胃镜检查联合组织miR-21表达水平检测对胃癌诊断的意义.方法 纳入2011年6月至2012年12月进行胃镜检查的患者96例,胃镜检查同时胃镜下钳取患者胃内病变组织,通过对比胃镜诊断与病理诊断结果计算胃镜确诊率及漏诊率;应用实时荧光定量PCR对组织进行miR-21表达水平检测,对比胃镜下不同表现的各种疾病的miR-21表达水平.结果 胃癌、胃溃疡、慢性浅表性胃炎、胃食管反流性胃炎、慢性萎缩性胃炎检出率分别为28.12％（27/96）、30.21％（29/96）、18.75％（18/96）、10.42％（10/96）、12.50 ％（12/96）,胃镜检查胃癌确诊率为100.00％,漏诊率6.90％（2/29）;胃癌组miR-21相对表达量为54.41±6.66,明显高于胃溃疡组及其他疾病组（P＜0.01）.结论 电子胃镜检查能较好地对胃癌患者进行筛查,胃镜下钳取组织行miR-21表达水平检测能提高胃镜对胃癌筛查的准确率,两者联合对胃癌诊断具重要价值.
Objective To evaluate the significance of electronic gastroscopy examination combined with miR-21 in the diagnosis of gastric carcinoma.Methods 96 gastroscopy cases in Guangzhou General Hospital of Guangzhou Military Command were adopted from June,2011 to December,2012.The patients received gastric endoscopy examination,and the diseased tissue samples were taken out at the same time.To calculate the diagnosis rate and missed rate with gastroscopy,by comparing the gastroscopy diagnosis with the pathology diagnosis.To evaluate the expression level of miR-21 of the tissue by applying quantitative real time PCR.Results The diagnosis rates of gastric cancer,gastric ulcer,chronic superficial gastritis,gastroesophageal reflux gastritis,chronic atrophic gastritis under gastroscopy were 28.12 ％ （27/96）,30.21％ （29/96）,18.75 ％ （18/96）,10.42 ％ （10/96）,12.50 ％ （12/96）.The diagnosis rate of gastric cancer was 100.00 ％ and the missed diagnosis rate was 6.90 ％ （2/29）,the relative transcript level of miR-21 in the gastric cancer group was 54.41±6.66,which was obviously higher than the gastric ulcer group and the other groups （P ＜ 0.01）.Conclusions Electronic gastroscopy is a clinically practical examination method in screening gastric cancer cases,evaluating the expression level of miR-21 in the diseased tissue under gastroscopy can enhance the accuracy of screening gastric cancer.Combining the two methods is useful in the diagnosis of gastric cancer.
出处
《肿瘤研究与临床》
CAS
2014年第7期454-457,共4页
Cancer Research and Clinic
基金
广东省科技计划项目（2011B031800317）
关键词
胃肿瘤
胃溃疡
电子胃镜
MIR-21
Stomach neoplasms
Gastric ulcer
Electronic gastroscopy
miR-21
