摘要
目的探讨核因子-κB(nuclear factor-κB,NF-κB)活性抑制剂SN50对大鼠视网膜缺血再灌注损伤(retinal ischemical reperfusion injury,RIRI)的保护作用。方法 27只成年雄性SD大鼠随机分为正常对照组、RIRI组、RIRI+SN50组,每组9只,建立RIRI动物模型,RIRI后6h、24h、72hHE染色观察大鼠视网膜病理变化,免疫组织化学检测NF-κB的表达,实时定量PCR检测TNF-α的表达。结果 RIRI组RIRI 6h后视网膜出现组织轻度水肿,少量细胞变性,随着时间的延长,视网膜的损伤逐渐加重;RIRI+SN50组视网膜的损伤明显减轻。RIRI后6h视网膜上可见NF-κB阳性表达,经过SN50注射处理后,NF-κB的阳性表达在RIRI后24h明显减弱;经过SN50注射处理后,与RIRI组比较,24h后视网膜的TNF-α表达差异有统计学意义(P<0.05)。结论 SN50对RIRI具有保护作用。
Objective To investigate the protective effects of nuclear factor-κB （NF-κB） inhibitor SN50 on retinal ischemia reperfusion injury （RIRI） in rats. Methods TwenW-seven male SD rats were randomly divided into the normal control group, RIRI group and RIRI ＋ SN50 group ,9 rats in each group. RIRI models were established in rats of RIRI group and RIRI ＋ SN50 group. Pathological changes of rat retina were observed at 6 hours,24 hours and 72 hours after RIRI,NF-κB expression sites were detected by immunohistochemistry, and the expression of TNF-α were detected by real-time PCR. Results At 5 hours after RIRI, the retinal tissue was edema slightly, a small amount of cells were degeneration. With the extension of rime,the retinal injury aggravated gradually. However, after injection of SN50, the retinal injury reduced significantly. The positive expression of NF-κB was observed in retina at 5 hours after RIRI. The positive expression of NF-κB significantly decreased after injection of SN50 at 24 hours after RIRI. Compared with RIRI group, the difference of TNF-α expression in RIRI ＋ SN50 group were statistically significant （P 〈 0.05 ）. Conclusion SN50 have the protective effects on RIRI.
出处
《眼科新进展》
CAS
北大核心
2014年第9期805-808,共4页
Recent Advances in Ophthalmology
基金
国家自然科学基金项目(编号:81200719)~~
