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酵母CFD1基因缺失菌株构建及其对复制寿命的影响 被引量：4

Construction of CFD1 deletion yeast strain and its effect on replicative life span
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摘要 目的测定CFD1基因缺失对酿酒酵母复制寿命的影响。方法运用基因同源重组技术构建单基因缺失酵母菌株,利用光学显微镜检测酵母细胞的复制寿命。结果与野生型酵母菌株(29.29±8.71代对比,CFD1基因缺失酵母菌株复制寿命(27.27±8.01)代有所变短,但差异无统计学意义。结论 CFD1基因缺失不影响酿酒酵母的复制寿命。) Objective To detect the effect of CFD1 deletion yeast strain on the replicative life span. Methods The CFD1 deletion yeast strain was constructed using gene homologous recombination, and the replicative life span was determined by light microscopy. Results The replicative life span showed no significant difference between CFD1 deletion and wide type yeast strains（27.27 vs 29.29, P〉0.05）. Conclusion The CFD1 deletion has no impact on the replicative life span of Saccharomyces cerevisiae.
作者 袁源 赵炜 崔红晶 刘新光
机构地区 广东医学院衰老研究所 广东省医学分子诊断重点实验室 广东医学院生物化学与分子生物学研究所
出处 《广东医学院学报》 2014年第3期280-281,286,共3页 Journal of Guangdong Medical College
基金 国家自然科学基金(No.31101051) 广东医学院建博科技创新团队项目(No.STIF201102) 广东医学院青年基金项目(No.XQ1212)
关键词 酿酒酵母 CFD1 复制寿命 Saccharomyces cerevisiae CFD1 replicative life span
分类号 Q291 [生物学—细胞生物学]
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参考文献9

  • 1Pillai V B, Sundaresan N R, Qupta M E Regulation of Akt signaling by sirtuins:its implication in cardiac hypertrophy and aging[J]. Circ Res, 2014, 114(2):368-378.
  • 2Netz D J, Pierik A J, St ti mpfig M, et al. A bridging [4Fe-4S] cluster and nucleotide binding are essential for function of the Cfdl-Nbp35 complex as a scaffold in iron-sulfur protein maturation [J]. J Biol Chem, 2012, 287(15):12365-12378.
  • 3Pallesen L J, Solodovnikova N, Sharma A K, et al. Interaction with Cfdl increases the kinetic lability of FeS on the Nbp35 scaffold [J]. J Biol Chem, 2013, 288(32):23358-23367.
  • 4方炳雄,赵炜,崔红晶,刘新光.酿酒酵母寿命的研究方法及进展[J].国际老年医学杂志,2013,34(1):28-34. 被引量：9
  • 5Cai L, McCormick M A, Kennedy B K, et al. Integration of multiple nutrient cues and regulation of lifespan by ribosomal transcription factor lfhl [J]. Cell Rep, 2013, 4(6):1063-1071.
  • 6Stehling O, Lill R. The role of mitochondria in cellular iron-sulfur protein biogenesis: mechanisms,connected processes, and diseases[J]. Cold Spring Harb Perspect Med, 2013, 3(7):1-17.
  • 7Sharma A K, Pallesen L J, Spang R J, et al. Cytosolic iron-sulfur cluster assembly (CIA) system:factors, mechanism, and relevance to cellular iron regulation[J]. J Biol Chem, 2010, 285(35):26745-26751.
  • 8Banci L, Bertini I, Calderone V, et al. Molecular view of an electron transfer process essential for iron-sulfur protein biogenesis[J]. Proc Natl Acad Sci USA, 2013, 110(18):7136-7141.
  • 9Hacioglu E, Demir A B, Koc A. Identification of respiratory chain gene mutations that shorten replicative life span in yeast[J]. Exp Gerontol, 2012, 47(2): 149-153.

二级参考文献41

  • 1Fontana L,Partridge L,Loungo VD. Extending healthy life span-from yeast to humans[J].Science,2010,(5976):321-326.
  • 2Steinkraus KA,Kaeberlein M,Kennedy BK. Replicative aging in yeast:the means to the end[J].Annual Review of Cell and Developmental Biology,2008.29-54.
  • 3Goffeau A,Barrell BG,Bussey H. Life with 6000 genes[J].Science,1996,(5287):546,563-567.
  • 4Benders GA,Noskov VN,Denisova EA. Cloning whole bacterial genomes in yeast[J].Nucleic Acids Research,2010,(08):2558-2569.
  • 5Norris A,Boeke JD. Silent information regulator 3:the Goldilocks of the silencing complex[J].Genes and Development,2010,(02):115-122.
  • 6Burtner CR,Murakami C J,Kennedy BK. A molecular mechanism of chronological aging in yeast[J].Cell Cycle,2009,(08):1256-1270.
  • 7Longo VD,Fabrizio P. Chronological aging in Saccharomyces cerevisiae[J].Sub-Cellular Biochemistry,2012.101-121.
  • 8Steinkraus KA,Kaeberlein M,Kennedy BK. Recent developments in yeast aging[J].PLoS Genetics,2007,(05):e84.
  • 9Lundblad V,Hartzog G,Moqtaderi Z. Manipulation of cloned yeast DNA[J].Current Protocols in Molecular Biology,2001.Chapter13Unit13.10.
  • 10Benders GA. Cloning whole bacterial genomes in yeast[J].Methods in Molecular Biology,2012.165-180.

共引文献8

同被引文献28

  • 1Girrbach V, Strahl S. Members of the evolutionarily conserv- ed PMT family of protein O-mannosyltransferases form distinct protein complexes among themselves [J]. J Biol Chem, 2003, 278(14): 12554-12562.
  • 2Goder V, Melero A. Protein O-mannosyltransferases partici- pate in ER protein quality control [J]. J Cell Sei, 2011, 124 (Pt 1): 144-153.
  • 3Lussier M, Gentzsch M, Sdicu A M, et al. Protein O-gly- cosylation in yeast. The PMT2 gene specifies a second pro- tein O-mannosyltransferase that functions in addition to the PMTl-encoded activity [J]. J Biol Chem, 1995, 270(6): 2770-2775.
  • 4Gentzsch M, Tanner W. The PMT gene family: protein O- glycosylation in Saccharomyces eerevisiae is vital [J]. EMBO J, 1996, 15(21): 5752-5759.
  • 5Strahl-Bolsinger S, Immervoll T, Deutzmann R, et al. PMT1, the gene for a key enzyme of proteiO-glycosylation in Sac- charomyces cerevisiae [J]. Proc Natl Acad Sci U S A, 1993,90(17): 8164-8168.
  • 6Zhao W, Fang B X, Niu Y J, et al. Narl deficiency results in shortened lifespan and sensitivity to paraquat that is rescued by increased expression of mitochondrial superoxide dismu- tase [J]. MechAgeing Dev, 2014, 138:53-58.
  • 7Baudin A, Ozier-Kalogeropoulos O, Denouel A, et al. A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae [J]. Nucleic Acids Res, 1993, 21(14): 3329-3330.
  • 8Kaeberlein M, McVey M, Guarente L. The SIR2/3/4 complex and SIR2 alone promote longevity in Saccharo- myces cerevisiae by two different mechanisms [J]. Genes Dev, 1999, 13(19): 2570-80.
  • 9Steffen K K, Kennedy B K, Kaeberlein M. Measuring replicative life span in the budding yeast [J]. J Vis Exp, 2009, 25(28): 1209.
  • 10Gentzsch M, Immervoll T, Tanner W. Protein O-glycosyla- tion in Saccharomyces cerevisiae: the protein O-maunosyl- transferases Pmtlp and Pmt2p function as heterodimer [J]. FEBS Lett, 1995, 377(2): 128-130.

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广东医学院学报
2014年 第3期

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