摘要
目的初步探讨贝母辛对内毒素诱导小鼠急性肺损伤(acute lung injury,ALI)保护作用及其可能机制。方法用乙醚麻醉小鼠,经鼻腔滴注脂多糖(lipopolysaccharide,LPS)(1 mg/5 ml PBS,每只50μl),构建小鼠急性肺损伤模型。将小鼠随机分为对照组、LPS组和贝母辛+LPS组。观察各组小鼠血清LDH、MDA含量,肺组织病理学改变,肺泡灌洗液上清总蛋白、白细胞计数及其分类计数。结果 LPS处理小鼠可明显上调血清LDH、MDA表达(P<0.01);LPS组肺组织病理学显示:气道上皮脱落,气道内有粘液分泌,肺泡结构呈实变,有大量炎症细胞渗出;LPS组肺泡灌洗液上清总蛋白、白细胞计数明显高于正常对照组(P<0.01)。贝母辛组均可有效降低急性肺损伤小鼠血清的LDH、MDA的表达(具有剂量依赖性),高剂量贝母辛组可明显减轻LPS所致肺组织病理学变化,减少粘液分泌;降低肺泡灌洗液中总蛋白、白细胞总数及淋巴细胞和中性粒细胞分类计数。结论贝母辛可减轻LPS所致急性肺组织损伤。
Objective To determine the protective effect of Peimisine on acute lung injury（ ALI） induced by lipopolysaccharide（ LPS） and its protective mechanism mice. Methods The mice were randomly allocated into sham,LPS and Peimisine ＋ LPS groups. The ALI mice was induced by LPS after etherization. The Peimisine was injected by belly cavity 30 minutes prior to the LPS challenge,one time/d,3 times totally. The mice were killed 24h after the third Peimisine injection to observe the amount of LDH,MDA in plasma,the lung tissue pathology,the total protein,white blood cell and differential count in the bronchoalveolar lavage fluid（ BALF）. Results LDH,MDA amount in plasma,Lung tissue,and BALF showed serious inflammatory changes in the LPS group. Compared with the LPS group,Peimisine attenuates Lung tissue injury,LDH and MDA amount in ALI mice in a dose dependent manner. Peimisine（ 0. 12mg） lowered the total protein,total white blood cells,lymphocyte and neutrophilic leukocyte in BALF compared with the LPS group. Conclusion Peimisine can play a protective role against LPS-induced acute lung injury.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2014年第9期2118-2120,共3页
Lishizhen Medicine and Materia Medica Research
基金
江西省卫生厅中医药科研基金(No.2012A151)
江西中医药大学重点学科基金和江西中医药大学道地药材专项基金
关键词
贝母辛
急性肺损伤
内毒素
保护作用
Peimisine
Acute lung injury
Lipopolysaccharide
Protective effect
