摘要
目的探讨脂联素对RAW264.7巨噬细胞源性泡沫细胞ABCA1及胆固醇含量的影响及其可能的机制。方法体外培养RAW264.7细胞,加入20 mg/L氧化低密度脂蛋白(ox-LDL)共同孵育48 h,将其诱导成泡沫细胞,加入不同浓度(0、1、5、10μg/mL)的脂联素干预24 h,RT-PCR测定ABCA1 mRNA的表达,高效液相色谱测定细胞内胆固醇含量。观察脂联素对泡沫细胞中ABCA1表达的影响。结果脂联素显著增加RAW264.7巨噬细胞源性泡沫细胞ABCA1 mRNA的表达(P<0.05),并增加细胞内胆固醇含量,且呈浓度依赖性(P<0.05)。结论脂联素可以增加巨噬源性泡沫细胞ABCA1转录水平,促进胆固醇流出,延缓AS的发生发展。
Objective Effects of adiponectin on RAW264.7 macrophage derived foam cells and cholesterol content to ABCA1） objective and its possible mechanisms. Methods RAW264.7 cells cultured in vitro, joined the 20mg/L oxidized low density lipoprotein（ox-LDL） were incubated for 48 h, the induced into foam cells, with different concentrations（0,1,5,10 μg/mL） of adiponectin 24 h expression of RT-PCR ABCA1 mRNA, determination, HPLC determination of intracellular cholesterol content. To observe the effects of adiponectin on the expression of ABCA1 in foam cells. Results The expression of adiponectin was significantly increased in RAW264.7 macrophage derived foam cells in ABCA1 mRNA（P〈0.05）, and increased cellular cholesterol content, in a dose-dependent manner（P〈0.05）. Conclusion Adiponectin can increase the macrophage derived foam cell ABCA1 transcription level, promote cholesterol efflux, delay the occurrence and development of AS.
出处
《中国医药指南》
2014年第25期46-48,共3页
Guide of China Medicine
