摘要
目的探讨新疆地区慢性乙型肝炎患者乙型肝炎病毒(HBV)耐药变异类型与基因型的关系。方法应用聚合酶链反应(PCR)对245例HBV DNA阳性标本进行HBV基因分型。采用基因芯片技术对HBV拉米夫定及阿德福韦酯相关耐药基因突变位点(包括180、204、181、236位点)进行检测。结果 245例慢性乙型肝炎患者检出HBV基因型B型40例、C型137例、C/D混合型48例、其它型别20例,在不同性别及年龄间的分布差异无统计学意义(χ2=1.791,P=0.617;χ2=14.214,P=0.115),而在不同民族间的分布差异有统计学意义(χ2=83.076,P=0.000)。HBV在180、204、181、236位点的突变检出率分别为33.8%、76.8%、66.9%、45.0%,差异有统计学意义(P<0.05);HBV常见变异类型的基因型构成差异无统计学意义(χ2=14.969,P=0.454),但C型HBV在各变异类型中所占的比例均最高。结论 HBV基因型在不同民族间的分布不同;HBV常见耐药位点的突变情况不同;HBV基因型在常见变异类型中的分布相同。
Objective To investigate the relationship between hepatitis B virus (HBV)mutational pattern and genotypes among chronic hepatitis B patients in Xinjiang.Methods Genotyping of 245 HBV DNA positive samples was performed by polymerase chain reaction (PCR).Lamivudine and adefovir associated resistant sites (1 80,204,1 81 and 236 sites)were analyzed by gene chips.Results There were 40 cases of HBV genotype B,1 37 cases of HBV genotype C,48 cases of HBV genotype C /D and 20 cases of other HBV genotype among the 245 chronic hepatitis B patients.The distribution was not significant statistically in different sex and ages (χ2 =1 .791 ,P =0.61 7;χ2 =1 4.21 4,P =0.1 1 5),but there was statistical significance in different ethnicities (χ2 =83.076,P =0.000).The mutational detection rates for HBV at 1 80,204,1 81 and 236 sites were 33.8%,76.8%,66.9% and 45.0%,with statistical significance (P <0.05).The distribution of HBV genotypes among the common mutational pattern had no statistical difference(χ2 =1 4.969,P =0.454),but HBV genotype C showed the highest proportion among the common mutational pattern.Conclusions HBV genotypes have different distribution in different ethnicities.The mutational information in common resistant sites is different.The distribution of HBV genotypes is not different in common mutational pattern.
出处
《检验医学》
CAS
2014年第10期1041-1044,共4页
Laboratory Medicine
关键词
乙型肝炎病毒
耐药
突变
基因型
汉族
维吾尔族
Hepatitis B virus
Drug resistance
Mutation
Genotype
Han population
Uighur population
