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自噬诱导剂海藻糖对帕金森症小鼠的治疗作用 被引量：3

The Effect of Trehalose, an Autophagy Enhancer,in the MPTP Model of Parkinson's Disease
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摘要 目的:探讨自噬诱导剂海藻糖对帕金森症小鼠的治疗作用。方法:使用1-甲基-4-苯基-1,2,3,6-四氢吡啶盐酸盐(MPTP)建立小鼠帕金森症模型,通过观察小鼠中脑黑质区多巴胺(DA)神经元数量、纹状体内神经递质多巴胺及代谢产物变化,判断海藻糖治疗帕金森症的作用。结果:(1)免疫组化染色发现MPTP使小鼠黑质区DA神经元数量明显减少。海藻糖干预后可增加DA神经元数量。(2)HPLC检测发现MPTP造成小鼠纹状体中多巴胺明显减少,海藻糖组可增加神经递质及代谢物。结论:海藻糖能减少MPTP造成的黑质区DA神经元丢失,增加纹状体中多巴胺的含量,海藻糖能有效治疗帕金森症。 Objective： To explore the effect of trehalose in the MPTP model of Parkinson’s disease. Methods： The number of surviving dopaminergic （DA） neurons in substantia nigra pars compacta （SNpc） and levels of dopamine and its main metabolites, 3,4-dihydroxyphenylacetic acid （DOPAC） and homovanillic acid （HVA） in striatum were evaluated. Re-sults： Trehalose rescued midbrain DA neurons from MPTP toxicity and increased the levels of dopamine, DOPAC, and HVA in striatum. Conclusion：Trehalose could be used for treatment of Parkinson’s disease.
作者 吴锋 秦巨峰
机构地区 南通大学药学院药理系 南通大学附属医院影像科
出处 《交通医学》 2014年第4期316-317,321,共3页 Medical Journal of Communications
基金 国家青年科学基金项目(31300893) 南通市科技项目(BK2012094)
关键词 帕金森症 自噬 海藻糖 1-甲基-4-苯基-1 2 3 6-四氢吡啶盐酸盐 免疫组织化学法 小鼠 Autophagy Trehalose Parkinson’s disease MPTP
分类号 R742.5 [医药卫生—神经病学与精神病学]
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参考文献8

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同被引文献51

  • 1Alfonso Schiavib,Natascia Ventura. The interplay between mitochondria and autophagy and its role in the aging process[J]. Experimental Gerontology, 2014,56 (8): 147- 153.
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  • 7Chang Hwa Jung, Seung-hyun Ro, Jing Cao, et al. mTOR regulation of autophagy[J]. FEBS Lett, 2010,584 (7): 1287-1295.
  • 8Wang Chen,Zhang Xiong,Teng Zhi-peng,et al. Downregulation of PI3K/Akt/mTOR signaling pathway in curcumin-indueed autophagy in APP/PS 1 double transgenic mice [J]. European J Pharmacology, 2014,740 (5): 312-320.
  • 9Zhong Yun,Wang Qing-jun, Li Xian-ting, et al. Distinct regulation of autophagic activity by Atg14L and rubicon associated with Beclinl-phosphatidylinositol-3-kinase complex[J]. Nat Cell Biol,2009,11 (4):468-476.
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交通医学
2014年 第4期

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