摘要
目的:分析胃癌组织中环指蛋白180(the ring finger protein,RNF180)与伴有Kazal域的富含半胱氨酸的逆转诱导蛋白(reversioninducing cysteine-rich protein with Kazal motif,RECK)基因的表达水平及与预后的关系.方法:应用Western blot及逆转录PCR(reverse transcription-PCR,RT-PCR)方法检测胃癌、癌旁组织及其正常胃黏膜中RNF180及RECK的表达水平,分析其表达水平变化与胃癌发生发展的关系.结果:RNF180在胃癌组织及癌旁组织中的表达水平较正常胃黏膜中显著下调(68.7%vs 6.1%,28.4%vs 6.1%,P<0.05);RECK在胃癌组织及癌旁组织中的表达显著低于正常胃黏膜(71.4%vs 5.3%,31.6%vs 5.3%,P<0.05).结论:RNF180及RECK在胃癌组织中表达水平显著降低,提示其可能在胃癌的发生发展过程中有重要作用.
AIM： To detect the expression of ring finger protein 180（RNF180） and reversion-inducing-cysteine-rich protein with Kazal motifs（RECK） in gastric carcinoma. METHODS： Western blot and reverse tran-scription-polymerase chain reaction（RT-PCR） were used to detect the expression of RECK and RNF180 in gastric cancer, pericarcinomatous tis-sue and normal gastric mucosal tissue. RESULTS： The positive rates of RNF180 expres-sion in gastric cancer and pericarcinomatous tis-sue were significantly lower than that in normal gastric mucosal tissue（68.7%, 28.4% 6.1%, P〈0.05）. RECK expression was significantly lowerin gastric carcinoma and pericarcinomatous tis-sue than in normal gastric mucosal tissue（71.4%, 31.6% vs 5.3%, P〈0.05）. CONCLUSION： RNF180 and RECK expression in gastric cancer is significantly reduced, sug-gesting that they may play an important role in the development of gastric cancer.
出处
《世界华人消化杂志》
CAS
北大核心
2014年第24期3660-3664,共5页
World Chinese Journal of Digestology
