摘要
本文采用单因素设计,进行均匀设计实验,用超声波提取法考察了溶剂种类、超声波强度、超声波提取时间、料液比(g/mL)对构树种子油提取率的影响,同时研究了构树种子油清除羟基自由基和超氧阴离子自由基的能力。结果表明,在以丙酮作提取剂,各因素对构树种子油提取效率的影响次序为:超声波强度>料液比>超声时间;工艺参数为:超声波强度为65%,料液比(g/mL)1∶12,超声提取时间7 min,构树种子油提取率可达30.2%。同时,以构树种子油清除羟基自由基能力和超氧阴离子自由基能力为指标,研究构树种子油的抗氧化能力。通过DPPH法和邻苯三酚自氧化法测定其抗氧化活性,结果发现其对羟基自由基的抑制率高达93.56%,而对超氧阴离子没有明显抑制作用。
In this study, the effects of extraction solvents, ultrasonic power, treatment time and ratio of solid to liquid were investigated by an uniform design experiment. Meanwhile, the scavenging activities of Broussonetia papyrifer seed oil on hydroxyl free radical and superoxide anion radical were determined. The results indicated that the influencing factors on extraction yield ofB. papyrifer seed oil were in this order （from strong to weak） ：ultrasonic power,ratio of solid to liq- uld,txeatment time. The optimized processing parameters were determined to be： ultrasonic power was 65%, treatment time was 7 rain, ratio of solid to liquid was I： 12. Under the optimized extraction conditions, the extraction yield of B. pa- Iryrifer oll was 30.2%. The antioxidant activities of the oil were determined by DPPH and pyrogallol autoxidation assays. The results indicated that B. papyrifer seed oil had strong hydroxyl free radical scavenging activity,reaching 93;56% ,but no superoxide anion radical scavenging activity.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2014年第10期1685-1689,共5页
Natural Product Research and Development
基金
大连市科学技术项目资助项目(2010J21DW027)
大学生创新创业训练项目资助项目(X2012192)
关键词
构树种子油
超声波强化
提取率
羟基自由基
超氧阴离子自由基
抗氧化性
Broussonetia papyrifer seed oil
ultrasonic power
extraction yield
hydroxyl free radical
superoxide anion freeradical
antioxidant activity
